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Serum Bile Acids Profiling in Inflammatory Bowel Disease Patients Treated with Anti-TNFs
- Source :
- Cells, Vol 8, Iss 8, p 817 (2019), Cells, Volume 8, Issue 8
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Background: Inflammatory bowel diseases (IBD), ulcerative colitis (UC), and Crohn&rsquo<br />s disease (CD), represent systematic chronic conditions with a deficient intestinal absorption. We first attempt to investigate the serum bile acids (sBAs) profile in a large cohort of IBD patients to evaluate changes under anti-TNF alpha treatment. Methods: Forty CD and 40 UC patients were enrolled and BAs were quantified by high-pressure liquid chromatography-electrospray-tandem mass spectrometry (HPLC-ES-MS/MS). Up to 15 different sBAs concentrations and clinical biomarkers where added to a Principal Component Analysis (PCA) to discriminate IBD from healthy conditions and treatment. Results: PCA allowed a separation into two clusters within CD (biologic-free patients and patients treated with anti-TNF alpha drugs and healthy subjects) but not UC. The first included CD. CD patients receiving anti-TNF alpha have an increase in total sBAs (4.11 1.23 &mu<br />M) compared to patients not exposed. Secondary BAs significantly increase after anti-TNF alpha treatment (1.54 0.83 &mu<br />M). Furthermore, multivariate analysis based on sBA concentration highlighted a different qualitative sBAs profile for UC and CD patients treated with conventional therapy. Conclusion: According to our results, anti-TNF alpha in CD restores the sBA profile by re-establishing the physiological levels. These findings indicate that, secondary BAs might serve as an indirect biomarker of the healing process.
- Subjects :
- Adult
Crohn’s disease
medicine.medical_specialty
digestive system
Inflammatory bowel disease
Gastroenterology
Article
Intestinal absorption
Bile Acids and Salts
Young Adult
03 medical and health sciences
0302 clinical medicine
Crohn Disease
Internal medicine
medicine
Humans
lcsh:QH301-705.5
Aged
ulcerative colitis
bile acids
Crohn's disease
Tumor Necrosis Factor-alpha
business.industry
secondary bile acids
digestive, oral, and skin physiology
Healthy subjects
Inflammatory Bowel Diseases
General Medicine
Middle Aged
medicine.disease
Ulcerative colitis
digestive system diseases
Large cohort
Treatment Outcome
lcsh:Biology (General)
Biomarker (medicine)
Colitis, Ulcerative
030211 gastroenterology & hepatology
business
absorption
Biomarkers
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 20734409
- Volume :
- 8
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Cells
- Accession number :
- edsair.doi.dedup.....513550b11ea5cf37b61b2eba6c8a3b82