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Dorsally derived netrin 1 provides an inhibitory cue and elaborates the'waiting period' for primary sensory axons in the developing spinal cord
- Source :
- Development. 133:1379-1387
- Publication Year :
- 2006
- Publisher :
- The Company of Biologists, 2006.
-
Abstract
- Dorsal root ganglion (DRG) neurons extend axons to specific targets in the gray matter of the spinal cord. During development, DRG axons grow into the dorsolateral margin of the spinal cord and projection into the dorsal mantle layer occurs after a `waiting period' of a few days. Netrin 1 is a long-range diffusible factor expressed in the ventral midline of the developing neural tube, and has chemoattractive and chemorepulsive effects on growing axons. Netrin 1 is also expressed in the dorsal spinal cord. However, the roles of dorsally derived netrin 1 remain totally unknown. Here, we show that dorsal netrin 1 controls the correct guidance of primary sensory axons. During the waiting period, netrin 1 is transiently expressed or upregulated in the dorsal spinal cord, and the absence of netrin 1 results in the aberrant projection of sensory axons, including both cutaneous and proprioceptive afferents, into the dorsal mantle layer. Netrin 1 derived from the dorsal spinal cord, but not the floor plate, is involved in the correct projection of DRG axons. Furthermore,netrin 1 suppresses axon outgrowth from DRG in vitro. Unc5crcm mutant shows abnormal invasion of DRG axons as observed in netrin 1 mutants. These results are the first direct evidence that netrin 1 in the dorsal spinal cord acts as an inhibitory cue for primary sensory axons and is a crucial signal for the formation of sensory afferent neural networks.
- Subjects :
- Indoles
animal structures
Sensory system
Biology
UNC5C
Models, Biological
Cell Line
Mice
Organ Culture Techniques
Dorsal root ganglion
Pregnancy
Cricetinae
Ganglia, Spinal
Netrin
medicine
Animals
Nerve Growth Factors
Neurons, Afferent
Mantle (mollusc)
Molecular Biology
In Situ Hybridization
Floor plate
Mice, Inbred ICR
Epidermal Growth Factor
Tumor Suppressor Proteins
fungi
Neural tube
Galactosides
Anatomy
Netrin-1
Spinal cord
Immunohistochemistry
Axons
medicine.anatomical_structure
Spinal Cord
nervous system
Mutation
embryonic structures
Female
Neuroscience
Developmental Biology
Subjects
Details
- ISSN :
- 14779129 and 09501991
- Volume :
- 133
- Database :
- OpenAIRE
- Journal :
- Development
- Accession number :
- edsair.doi.dedup.....5104096db3ea5475b5408e6a1de8ca16