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Chryseobacterium cucumeris sp. nov., an endophyte isolated from cucumber (Cucumis sativus L.) root, and emended description of Chryseobacterium arthrosphaerae
- Source :
- International Journal of Systematic and Evolutionary Microbiology. 67:610-616
- Publication Year :
- 2017
- Publisher :
- Microbiology Society, 2017.
-
Abstract
- The Gram-stain-negative, yellow-pigmented, rod-shaped bacterial strain GSE06T, isolated from the surface-sterilized root of a cucumber plant grown in a field in Gunsan, Korea, was characterized by not only cultural and morphological features but also physiological, biochemical and molecular analyses. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain GSE06T was most closely related to species of the genus Chryseobacterium . Furthermore, strain GSE06T exhibited the highest sequence similarities with the type strains Chryseobacterium indologenes ATCC 29897T (98.9 %), Chryseobacterium gleum ATCC 35910T (98.8 %), Chryseobacterium arthrosphaerae CC-VM-7T (98.7 %), Chryseobacterium contaminans C26T (98.5 %), Chryseobacterium artocarpi UTM-3T (98.3 %), and Chryseobacterium gallinarum 100T (97.9 %). Average nucleotide identity values between genome sequences of strain GSE06T and the above-mentioned reference strains ranged from 81.2 to 86.9 %, which were lower than the threshold of 95 % (corresponding to a DNA–DNA reassociation value of 70 %). The DNA G+C content of strain GSE06T was 36.1 mol%; the predominant respiratory quinone of the strain was MK-6. The major fatty acids were iso-C15 : 0, summed feature 9 (iso-C17 : 1ω9c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, three aminolipids, one aminophospholipid, four glycolipids and one unidentified lipid. These results of phenotypic and genotypic characteristics could differentiate strain GSE06T from closely related type strains belonging to the genus Chryseobacterium . Thus, strain GSE06T is proposed as a representative of a novel species in the genus Chryseobacterium, Chryseobacterium cucumeris sp. nov. The type strain is GSE06T (=KACC 18798T=JCM 31422T).
- Subjects :
- DNA, Bacterial
0301 basic medicine
Chryseobacterium indologenes
Chryseobacterium gleum
Biology
Plant Roots
Microbiology
Chryseobacterium contaminans
03 medical and health sciences
Chryseobacterium arthrosphaerae
RNA, Ribosomal, 16S
Republic of Korea
Botany
Endophytes
Phospholipids
Phylogeny
Ecology, Evolution, Behavior and Systematics
Chryseobacterium
Base Composition
Strain (chemistry)
Pigmentation
Fatty Acids
Nucleic Acid Hybridization
Vitamin K 2
Sequence Analysis, DNA
General Medicine
16S ribosomal RNA
Bacterial Typing Techniques
Chryseobacterium gallinarum
030104 developmental biology
Chryseobacterium artocarpi
Cucumis sativus
Subjects
Details
- ISSN :
- 14665034 and 14665026
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- International Journal of Systematic and Evolutionary Microbiology
- Accession number :
- edsair.doi.dedup.....51002a01d6f6cc45bd226bb9373f1ff3