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Intracellular delivery of oligonucleotides in Helicobacter pylori by fusogenic liposomes in the presence of gastric mucus
- Source :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP, BIOMATERIALS, Biomaterials
- Publication Year :
- 2017
-
Abstract
- The rising antimicrobial resistance contributes to 25000 annual deaths in Europe. This threat to the public health can only be tackled if novel antimicrobials are developed, combined with a more precise use of the currently available antibiotics through the implementation of fast, specific, diagnostic methods. Nucleic acid mimics (NAMs) that are able to hybridize intracellular bacterial RNA have the potential to become such a new class of antimicrobials and additionally could serve as specific detection probes. However, an essential requirement is that these NAMs should be delivered into the bacterial cytoplasm, which is a particular challenge given the fact that they are charged macromolecules. We consider these delivery challenges in relation to the gastric pathogen Helicobacter pylori, the most frequent chronic infection worldwide. In particular, we evaluate if cationic fusogenic liposomes are suitable carriers to deliver NAMs across the gastric mucus barrier and the bacterial envelope. Our study shows that DOTAP-DOPE liposomes post-PEGylated with DSPE-PEG (DSPE Lpx) can indeed successfully deliver NAMs into Helicobacter pylori, while offering protection to the NAMs from binding and inactivation in gastric mucus isolated from pigs. DSPE Lpx thus offer exciting new possibilities for in vivo diagnosis and treatment of Helicobacter pylori infections.
- Subjects :
- 0301 basic medicine
Cytoplasm
PEGYLATED LIPOPLEXES
Swine
Antibiotics
Oligonucleotides
02 engineering and technology
SINGLE-PARTICLE TRACKING
Polyethylene Glycols
Fatty Acids, Monounsaturated
Drug Delivery Systems
Anti-Infective Agents
Medicine and Health Sciences
INFECTIOUS-DISEASES
DRUG-DELIVERY
Pathogen
In Situ Hybridization, Fluorescence
Liposome
Stomach
RESISTANT STAPHYLOCOCCUS-AUREUS
food and beverages
Drug Resistance, Microbial
021001 nanoscience & nanotechnology
Antimicrobial
3. Good health
Lipoplexes
RNA, Bacterial
Mechanics of Materials
Drug delivery
0210 nano-technology
medicine.drug_class
Biophysics
Bioengineering
Biology
Infections
Microbiology
PHOSPHORODIAMIDATE MORPHOLINO OLIGOMERS
Helicobacter Infections
Biomaterials
03 medical and health sciences
SURFACE-CHEMISTRY
Antibiotic resistance
medicine
BARRIER PROPERTIES
Animals
Fluorescent Dyes
Nucleic acid mimics
Helicobacter pylori
Phosphatidylethanolamines
GASTROINTESTINAL MUCUS
Molecular Mimicry
Biology and Life Sciences
IN-SITU HYBRIDIZATION
Oligonucleotides, Antisense
biology.organism_classification
Mucus
Virology
Quaternary Ammonium Compounds
030104 developmental biology
RNA, Ribosomal
Liposomes
Ceramics and Composites
Gastric mucus
Subjects
Details
- Language :
- Portuguese
- ISSN :
- 01429612
- Database :
- OpenAIRE
- Journal :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP, BIOMATERIALS, Biomaterials
- Accession number :
- edsair.doi.dedup.....50fe9bd22fd1a51b2ebafb0f350d560f