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CD44 Promotes Intoxication by the Clostridial Iota-Family Toxins
- Source :
- PLoS ONE, PLoS ONE, Public Library of Science, 2012, 7 (12), pp.e51356. ⟨10.1371/journal.pone.0051356⟩, BASE-Bielefeld Academic Search Engine, PLoS ONE, Vol 7, Iss 12, p e51356 (2012), PLoS ONE, 2012, 7 (12), pp.e51356. ⟨10.1371/journal.pone.0051356⟩, PLoS ONE, Public Library of Science, 2012, 7 (12), pp.e51356. 〈10.1371/journal.pone.0051356〉
- Publication Year :
- 2012
- Publisher :
- HAL CCSD, 2012.
-
Abstract
- International audience; Various pathogenic clostridia produce binary protein toxins associated with enteric diseases of humans and animals. Separate binding/translocation (B) components bind to a protein receptor on the cell surface, assemble with enzymatic (A) component(s), and mediate endocytosis of the toxin complex. Ultimately there is translocation of A component(s) from acidified endosomes into the cytosol, leading to destruction of the actin cytoskeleton. Our results revealed that CD44, a multifunctional surface protein of mammalian cells, facilitates intoxication by the iota family of clostridial binary toxins. Specific antibody against CD44 inhibited cytotoxicity of the prototypical Clostridium perfringens iota toxin. Versus CD44(+) melanoma cells, those lacking CD44 bound less toxin and were dose-dependently resistant to C. perfringens iota, as well as Clostridium difficile and Clostridium spiroforme iota-like, toxins. Purified CD44 specifically interacted in vitro with iota and iota-like, but not related Clostridium botulinum C2, toxins. Furthermore, CD44 knockout mice were resistant to iota toxin lethality. Collective data reveal an important role for CD44 during intoxication by a family of clostridial binary toxins.
- Subjects :
- Bacterial Diseases
Mouse
Clostridium spiroforme
Cytotoxicity
[ SDV.TOX ] Life Sciences [q-bio]/Toxicology
lcsh:Medicine
Pathogenesis
medicine.disease_cause
Biochemistry
Mice
Clostridium
AB toxin
Chlorocebus aethiops
Clostridium botulinum
Toxins
lcsh:Science
ADP Ribose Transferases
Mice, Knockout
0303 health sciences
Multidisciplinary
biology
Animal Models
Clostridium difficile
Endocytosis
Recombinant Proteins
Bacterial Pathogens
3. Good health
Hyaluronan Receptors
Infectious Diseases
Medical Microbiology
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Medicine
Vero cells
Research Article
Bacterial Toxins
Blotting, Western
Intoxication
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Microbiology
03 medical and health sciences
Model Organisms
Cell Line, Tumor
medicine
Animals
Immunoprecipitation
Protein Interactions
Biology
030304 developmental biology
Gram Positive
Dose-Response Relationship, Drug
[ SDV.BC ] Life Sciences [q-bio]/Cellular Biology
030306 microbiology
Toxin
lcsh:R
Proteins
CD coreceptors
biology.organism_classification
Actin cytoskeleton
Dithiothreitol
Cytoskeletal Proteins
lcsh:Q
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Public Library of Science, 2012, 7 (12), pp.e51356. ⟨10.1371/journal.pone.0051356⟩, BASE-Bielefeld Academic Search Engine, PLoS ONE, Vol 7, Iss 12, p e51356 (2012), PLoS ONE, 2012, 7 (12), pp.e51356. ⟨10.1371/journal.pone.0051356⟩, PLoS ONE, Public Library of Science, 2012, 7 (12), pp.e51356. 〈10.1371/journal.pone.0051356〉
- Accession number :
- edsair.doi.dedup.....50e3b40e25092607454b2cb2ac519c75