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Validated UHPLC-HRMS method for simultaneous quantification of flavonoid contents in the aerial parts of Chenopodium bonus-henricus L. (wild spinach)
- Source :
- Pharmacia 68(3): 597-601, Pharmacia, Vol 68, Iss 3, Pp 597-601 (2021)
- Publication Year :
- 2021
- Publisher :
- Pensoft Publishers, 2021.
-
Abstract
- A UHPLC-HRMS method for simultaneous quantification of flavonoid contents in the aerial parts of Chenopodium bonus-henricus L. was developed and validated. The amount of 12 detected flavonoids was calculated relative to external standard hyperoside. The calibration curve of hyperoside showed very good linear regressions and the correlation coefficient was R2 > 0.9979. The limits of detection and quantitation limits were 0.39 ng/mL and 1.17 ng/mL, respectively. The UHPLC-HRMS method showed acceptable accuracy. At three different concentrations the recoveries of hyperoside ranging from 99.63% to 100.70% with RSD from 1.58% to 2.31%. The intra-day and inter-day precision were determined by analyzing the retention times and recovery of the external standard. The glycosides of spinacetin and patulenin (1) were the predominant compounds in the wild spinach which contents ranging from 1.79 to 4.41 mg g-1 D.W., calculated as hyperoside. The total amount of flavonoids was found to be 15.12 mg g-1 D.W.
- Subjects :
- Chenopodium bonus-henricus
Flavonoid
Uhplc hrms
Blitum bonus-henricus
Pharmaceutical Science
Pharmacy
Magnoliopsida
Pharmacy and materia medica
UHPLC-HRMS
Pharmacology (medical)
Plantae
chemistry.chemical_classification
Chenopodioideae
Amaranthaceae
Chromatography
biology
Blitum
biology.organism_classification
Biota
Caryophyllales
quantification
RS1-441
Tracheophyta
chemistry
flavonoids
Spinach
Subjects
Details
- ISSN :
- 2603557X and 04280296
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Pharmacia
- Accession number :
- edsair.doi.dedup.....50dcd4f982b04ec73c98bf8868b5ecdd