Coordinate effects of P2X7 and extracellular acidification in microglial cells

// Ponarulselvam Sekar 1 , Duen-Yi Huang 2 , Shwu-Fen Chang 1 and Wan-Wan Lin 1, 2 1 Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan 2 Department of Pharmacology, College of Medicine, National Taiwan University, Taipei, Taiwan Correspondence to: Wan-Wan Lin, email: wwllaura1119@ntu.edu.tw Keywords: P2X7; ATP; acidification; mitochondrial respiration; mitochondrial fission Received: April 20, 2017 Accepted: January 24, 2018 Published: January 29, 2018 ABSTRACT Extracellular adenosine 5′-triphosphate (ATP) is a damage-associated molecular pattern and contributes to inflammation associated diseases including cancer. Extracellular acidosis is a novel danger signal in the inflammatory sites, where it can modulate inflammation, immunity and tumor growth. Extracellular acidification was shown to inhibit P2X7-mediated channel currents, while it remains unknown how acidification and P2X7 together affect cellular responses. Here, we treated BV-2 microglial cells with ATP in a short period (