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A microfluidics-based screening tool to assess the impact of blood plasma factors on microvascular integrity
- Source :
- Advanced Biology, 5(11). WILEY-V C H VERLAG GMBH, Advanced Biology, 5(11):2100954. Wiley-VCH
- Publication Year :
- 2021
-
Abstract
- This study provides a method to assess the impact of circulating plasma factors on microvascular integrity by using a recently developed microvessel-on-a-chip platform featuring the human endothelium that is partly surrounded by the extracellular matrix. The system is high-throughput, which allows parallel analysis of organ-level microvessel pathophysiology, including vascular leakage. Ethylenediaminetetraacetic acid plasma samples are mixed with inhibitors for recalcification of the plasma samples to avoid activation of the coagulation- or complement system. Moreover, the assay is validated by spiking vascular endothelial growth factor, histamine, or tumor necrosis factor alpha to recalcified plasma and confirms their modulation of microvessel barrier function at physiologically relevant concentrations. Finally, this study shows that perfusing the microvessels with recalcified plasma samples of coronavirus disease-2019 patients, with a confirmed proinflammatory profile, results in markedly increased leakage of the microvessels. The assay provides opportunities for diagnostic screening of inflammatory or endothelial disrupting plasma factors associated with endothelial dysfunction.
- Subjects :
- Vascular Endothelial Growth Factor A
Pathology
medicine.medical_specialty
Endothelium
Microfluidics
Biomedical Engineering
General Biochemistry, Genetics and Molecular Biology
Proinflammatory cytokine
Biomaterials
Extracellular matrix
Plasma
chemistry.chemical_compound
SDG 3 - Good Health and Well-being
Blood plasma
medicine
Humans
Endothelial dysfunction
Microvessel
Barrier function
SARS-CoV-2
Chemistry
COVID-19
medicine.disease
Vascular endothelial growth factor
medicine.anatomical_structure
Endothelium, Vascular
Subjects
Details
- Language :
- English
- ISSN :
- 27010198
- Database :
- OpenAIRE
- Journal :
- Advanced Biology, 5(11). WILEY-V C H VERLAG GMBH, Advanced Biology, 5(11):2100954. Wiley-VCH
- Accession number :
- edsair.doi.dedup.....50d23080402f3649f686860ec736021b