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Streptococcus pneumoniae binds to host GAPDH on dying lung epithelial cells worsening secondary infection following influenza
- Source :
- Cell Reports, Vol 35, Iss 11, Pp 109267-(2021), Cell reports
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- SUMMARY Streptococcus pneumoniae (Spn) alone and during co-infection with influenza A virus (IAV) can result in severe pneumonia with mortality. Pneumococcal surface protein A (PspA) is an established virulence factor required for Spn evasion of lactoferricin and C-reactive protein-activated complement-mediated killing. Herein, we show that PspA functions as an adhesin to dying host cells. We demonstrate that PspA binds to host-derived glyceraldehyde-3-phosphate dehydrogenase (GAPDH) bound to outward-flipped phosphatidylserine residues on dying host cells. PspA-mediated adhesion was to apoptotic, pyroptotic, and necroptotic cells, but not healthy lung cells. Using isogenic mutants of Spn, we show that PspA-GAPDH-mediated binding to lung cells increases pneumococcal localization in the lower airway, and this is enhanced as a result of pneumolysin exposure or co-infection with IAV. PspA-mediated binding to GAPDH requires amino acids 230–281 in its α-helical domain with intratracheal inoculation of this PspA fragment alongside the bacteria reducing disease severity in an IAV/Spn pneumonia model.<br />In brief Park et al. demonstrate that pneumococcal surface protein A (PspA) acts as a bacterial adhesin. They show that PspA mediates Streptococcus pneumoniae binding to dying host cells via interactions with surface-bound host GAPDH. Pneumococcal localization in the lower airway is enhanced following pneumolysin- or influenza A virus (IAV)-mediated damage in a PspA-dependent manner.<br />Graphical abstract
- Subjects :
- 0301 basic medicine
super-infection
QH301-705.5
Secondary infection
PspA
Biology
medicine.disease_cause
Article
Protein Structure, Secondary
General Biochemistry, Genetics and Molecular Biology
Virulence factor
Microbiology
03 medical and health sciences
0302 clinical medicine
Bacterial Proteins
Influenza, Human
Streptococcus pneumoniae
medicine
Influenza A virus
Animals
Humans
pneumonia
Biology (General)
Lung
Pneumolysin
Cell Death
Coinfection
Glyceraldehyde-3-Phosphate Dehydrogenases
Epithelial Cells
medicine.disease
Mice, Inbred C57BL
Bacterial adhesin
Pneumonia
030104 developmental biology
A549 Cells
Apoptosis
Host-Pathogen Interactions
Female
influenza
pneumococcus
030217 neurology & neurosurgery
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 22111247
- Volume :
- 35
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Cell Reports
- Accession number :
- edsair.doi.dedup.....50c58fef238e0bafec37633902468e4b