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Elucidation of the FKBP25-60S Ribosomal Protein L7a Stress Response Signaling During Ischemic Injury
- Source :
- Cellular Physiology and Biochemistry, Vol 47, Iss 5, Pp 2018-2030 (2018)
- Publication Year :
- 2018
- Publisher :
- Cell Physiol Biochem Press GmbH & Co KG, 2018.
-
Abstract
- Background/Aims: Peptidyl-prolyl cis-trans isomerase FKBP25 is a member of the FK506-binding proteins family which has peptidyl-prolyl cis/trans isomerase domain. The biological function and pathophysiologic role of FKBP25 remain elusive. Methods: The spatio-temporal changes in expression of endothelial FKBP25 upon oxygen-glucose deprivation (OGD) treatment were examined by Western blot and immunofluorescence. The immunoprecipitation and fluorescence resonance energy transfer (FRET) were used to address the interacting proteins with FKBP25. Results: In the present study, nuclear translocation of FKBP25 was observed following OGD in cultured endothelial cells. Intriguingly, FKBP25 nuclear translocation was further validated in peroxynitrite (ONOO-)-treated endothelial cells. Coimmunoprecipitation and FRET data indicated that FKBP25 translocated into the nucleus, in which it interacted with 60S ribosomal protein L7a, while overexpression FKBP25 protect endothelial cells against OGD injury. Conclusion: Our findings reveal that the nuclear import of FKBP25 and binding with 60S ribosomal protein L7a are protective stress responses to ischemia/nitrosaive stress injury.
- Subjects :
- Ribosomal Proteins
0301 basic medicine
Physiology
Immunoprecipitation
Active Transport, Cell Nucleus
Nuclear translocation
Nitrosative stress
Oxygen-glucose deprivation
Immunofluorescence
lcsh:Physiology
Tacrolimus Binding Proteins
Stress Response Signaling
lcsh:Biochemistry
Mice
03 medical and health sciences
chemistry.chemical_compound
Western blot
Stress, Physiological
medicine
Animals
lcsh:QD415-436
Cell Nucleus
medicine.diagnostic_test
Fkbp25
lcsh:QP1-981
Chemistry
Ribosome Subunits, Large, Eukaryotic
60S Ribosomal Protein L7a
Cell Hypoxia
Cell biology
030104 developmental biology
Förster resonance energy transfer
60S ribosomal protein L7a
Nuclear transport
Peroxynitrite
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 14219778 and 10158987
- Volume :
- 47
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Cellular Physiology and Biochemistry
- Accession number :
- edsair.doi.dedup.....50bbc4a3c07602334920c4285cfe4817