Supplementary figure 1 from In Silico Analysis Guides Selection of BET Inhibitors for Triple-Negative Breast Cancer Treatment

A) JQ1 and OTX015 inhibitory action, as the IC50, calculated after 48 and 72 hours of treatment using MTT assay as described in "Material and methods". 2B) Antiproliferative effect of JQ1 in semi-solid MDA-MB-231 cultures. Cells (25,000/ml) were plated in 48 multiwell plates and grown in medium containing 2% matrigel in the presence of JQ1 (0.2µM), cisplatin (2.5 µM) and docetaxel (0.5nM) alone and in combination for 3 days. All images were taken at Ã-20 magnification. The quantification of sphere diameter was performed manually by tracing a straight line across the sphere diameter of untreated cells (controls) and scoring its value as arbitrary length units. Total number of colonies per plate was manually counted.