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Evidence for a new allele at the SERCA1 locus affecting pork meat quality in part through the imbalance of Ca2+ homeostasis
- Source :
- Molecular biology reports. 37(1)
- Publication Year :
- 2009
-
Abstract
- Sarcoendoplasmic reticulum Ca2+-ATPase 1 (SERCA1) as a Ca2+ release channel plays a key role in the relaxation of skeletal muscle through pumping cytosolic Ca2+ into the SR (sarcoplasmic reticulum). In this study, a novel single nucleotide polymorphism (SNP) in exon 8 (C > T) was detected by tetra-primer ARMS-PCR and the tissue expression pattern of SERCA1 was analyzed in eleven tissues. A model of primary skeletal muscle cells in vitro exposed to dexamethasone (DEX, a synthetic corticosteroid) was also employed to determine whether stress hormones cause an increase in intracellular Ca2+ concentration that is associated with alteration in SERCA1 and in turn subsequently affect meat quality. The results showed that the CC genotype has lower content intramuscular fat and higher water than pig carrying the genotype CT and CC. In addition, the additive effects were both significantly (P < 0.05) and allele T seemed to be associate with increase in intramuscular fat, while decrease in water content. Accompanied with previous studies, the high abundance of porcine SERCA1 was found in skeletal muscle tissue. DEX markedly down-regulated the expression of SERCA1, leading to Ca2+ overload. Furthermore, the imbalance of Ca2+ homeostasis up-regulated the transcription level of Calpain1. Taken together, we demonstrated a novel mechanism that the changes in expression of SERCA1 potential disturb the normal Ca2+ channel as well as the balance of Ca2+ homeostasis and which in turn finally activated Ca2+-dependent proteases such as Calpain1 which could affect meat quality.
- Subjects :
- medicine.medical_specialty
Meat
Genotype
Swine
Intracellular Space
Biology
Polymorphism, Single Nucleotide
Dexamethasone
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Stress, Physiological
Internal medicine
Gene expression
Genetics
medicine
Animals
Homeostasis
Muscle, Skeletal
Molecular Biology
Alleles
Calcium metabolism
Calpain
Endoplasmic reticulum
Gene Expression Profiling
Skeletal muscle
General Medicine
Endocrinology
medicine.anatomical_structure
Biochemistry
Gene Expression Regulation
Genetic Loci
Organ Specificity
Mutation
biology.protein
Calcium
Intramuscular fat
Hormone
Subjects
Details
- ISSN :
- 15734978
- Volume :
- 37
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Molecular biology reports
- Accession number :
- edsair.doi.dedup.....508194de6b6b1bb8967f17c2f8c8203b