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Effects of Prolyl Hydroxylase Inhibitor L-mimosine on Dental Pulp in the Presence of Advanced Glycation End Products
- Source :
- Journal of endodontics. 41(11)
- Publication Year :
- 2015
-
Abstract
- Introduction Proangiogenic prolyl hydroxylase (PHD) inhibitors represent a novel approach to stimulate tissue regeneration. Diabetes mellitus involves the accumulation of advanced glycation end products (AGEs). Here we evaluated the impact of AGEs on the response of human pulp tissue to the PHD inhibitor L-mimosine (L-MIM) in monolayer cultures of dental pulp–derived cells (DPCs) and tooth slice organ cultures. Methods In monolayer cultures, DPCs were incubated with L-MIM and AGEs. Viability was assessed based on formazan formation, live-dead staining, annexin V/propidium iodide, and trypan blue exclusion assay. Vascular endothelial growth factor (VEGF), interleukin (IL)-6, and IL-8 production was evaluated by quantitative polymerase chain reaction and immunoassays. Furthermore, expression levels of odontoblast markers were assessed, and alizarin red staining was performed. Tooth slice organ cultures were performed, and VEGF, IL-6, and IL8 levels in their supernatants were measured by immunoassays. Pulp tissue vitality and morphology were assessed by MTT assay and histology. Results In monolayer cultures of DPCs, L-MIM at nontoxic concentrations increased the production of VEGF and IL-8 in the presence of AGEs. Stimulation with L-MIM decreased alkaline phosphatase levels and matrix mineralization also in the presence of AGEs, whereas no significant changes in dentin matrix protein 1 and dentin sialophosphoprotein expression were observed. In tooth slice organ cultures, L-MIM increased VEGF but not IL-6 and IL-8 production in the presence of AGEs. The pulp tissue was vital, and no signs of apoptosis or necrosis were observed. Conclusions Overall, in the presence of AGEs, L-MIM increases the proangiogenic capacity, but decreases alkaline phosphatase expression and matrix mineralization.
- Subjects :
- Glycation End Products, Advanced
Pathology
medicine.medical_specialty
Cell Survival
Enzyme-Linked Immunosorbent Assay
Models, Biological
chemistry.chemical_compound
Organ Culture Techniques
stomatognathic system
Dentin sialophosphoprotein
Medicine
Humans
Mimosine
MTT assay
General Dentistry
Cells, Cultured
Dental Pulp
business.industry
Gene Expression Profiling
Prolyl-Hydroxylase Inhibitors
Molecular biology
Immunohistochemistry
Vascular endothelial growth factor
stomatognathic diseases
Odontoblast
chemistry
Pulp (tooth)
Advanced glycation end-product
Alkaline phosphatase
Cytokines
Trypan blue
business
Subjects
Details
- ISSN :
- 18783554
- Volume :
- 41
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of endodontics
- Accession number :
- edsair.doi.dedup.....506ef00c4d685c8bef784fa8da96aa92