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Improved gene transfer with histidine-functionalized mesoporous silica nanoparticles
- Source :
- International Journal of Pharmaceutics, International Journal of Pharmaceutics, Elsevier, 2014, pp.197-205. ⟨10.1016/j.ijpharm.2014.05.020⟩
- Publication Year :
- 2014
-
Abstract
- Mesoporous silica nanoparticles (MSN) were functionalized with aminopropyltriethoxysilane (MSN-NH2) then L-histidine (MSN-His) for pDNA delivery in cells and in vivo. The complexation of pDNA with MSN-NH2 and MSN-His was first studied with gel shift assay. pDNA complexed with MSN-His was better protected from DNase degradation than with MSN-NH2. An improvement of the transfection efficiency in cells was observed with MSN-His/pDNA compared to MSN-NH2/pDNA, which could be explained by a better internalization of MSN-His. The improvement of the transfection efficiency with MSN-His was also observed for gene transfer in Achilles tendons in vivo.
- Subjects :
- animal structures
media_common.quotation_subject
Genetic Vectors
Pharmaceutical Science
Nanoparticle
Cytomegalovirus
Transfection
Achilles Tendon
Mice
In vivo
Luciferases, Firefly
Animals
Humans
Electrophoretic mobility shift assay
Histidine
Internalization
ComputingMilieux_MISCELLANEOUS
media_common
Drug Carriers
Propylamines
[CHIM.ORGA]Chemical Sciences/Organic chemistry
Chemistry
Gene Transfer Techniques
DNA
Mesoporous silica
Silanes
Silicon Dioxide
3. Good health
HEK293 Cells
Biochemistry
Biophysics
Degradation (geology)
Nanoparticles
sense organs
Plasmids
Subjects
Details
- ISSN :
- 18733476 and 03785173
- Volume :
- 471
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- International journal of pharmaceutics
- Accession number :
- edsair.doi.dedup.....506cfa0a5ecf25d8c6a2c6d65058f6cd