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Hispolon inhibits RANKL induced osteoclast differentiation in vitro
- Source :
- Immunology letters. 231
- Publication Year :
- 2020
-
Abstract
- Hispolon (HISP) is a bioactive compound isolated from Phellinu linteus. It has various pharmacological activities, including antioxidant, anti-inflammatory, and anti-cancer. However, its anti-osteoclastogenic activity has not yet been reported. Hence, in the current study, we have explored the anti-osteoclastogenic activity of HISP and elucidated the molecular mechanisms. HISP inhibited the RANKL induced differentiation of RAW 264.7 cells into osteoclasts in a dose-dependent manner. Mechanistic studies showed that HISP inhibited RANKL-mediated activation of NF-κB and MAPK signaling pathways in osteoclast precursors RAW 264.7 cells. In addition, Hispolon also downregulated the expression of master transcriptional factors essential for osteoclast differentiation, such as NFATc1 and c-FOS. In conclusion, these findings establish molecular mechanisms behind the anti-osteoclastogenic activity of HISP.
- Subjects :
- musculoskeletal diseases
0301 basic medicine
Antioxidant
Cell Survival
MAP Kinase Signaling System
medicine.medical_treatment
Immunology
Catechols
Fluorescent Antibody Technique
Osteoclasts
Bone resorption
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Osteoclast
Osteogenesis
medicine
Immunology and Allergy
Animals
Transcription factor
Cells, Cultured
biology
Hispolon
RANK Ligand
Cell Differentiation
Bioactive compound
In vitro
Actins
Cell biology
030104 developmental biology
medicine.anatomical_structure
RAW 264.7 Cells
chemistry
RANKL
biology.protein
Reactive Oxygen Species
Biomarkers
030215 immunology
Subjects
Details
- ISSN :
- 18790542
- Volume :
- 231
- Database :
- OpenAIRE
- Journal :
- Immunology letters
- Accession number :
- edsair.doi.dedup.....506c9e3aff3216765bde0e5c81baa13a