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Long non-coding RNA LINC00525 interacts with miR-31–5p and miR-125a-5p to act as an oncogenic molecule in spinal chordoma
- Source :
- Biochemical and Biophysical Research Communications. 536:80-87
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- LINC00525 is a new-researched long non-coding RNA (lncRNA) in a few cancers. This study aims at researching the function of LINC00525 in spinal chordoma and the underlying mechanism of action. LINC00525, microRNA-31–5p (miR-31–5p) and microRNA-125a-5p (miR-125a-5p) detection was performed by quantitative real-time polymerase chain reaction (qRT-PCR). We found the high expression of LINC00525 but the low levels of miR-31–5p and miR-125a-5p in spinal chordoma tissues. After LINC00525 was downregulated in spinal chordoma cells, there were inhibitory effects on cell proliferation, migration, invasion and EMT but a promoting effect on cell apoptosis. MiR-31–5p and miR-125a-5p were the downstream targets of LINC00525. The function of LINC00525 knockdown in spinal chordoma cells were achieved by upregulating miR-31–5p and miR-125a-5p. Tumorigenesis of spinal chordoma in vivo was also inhibited by knockdown of LINC00525 via the promotion of miR-31–5p and miR-125a-5p. All these results suggested that LINC00525 targeted miR-31–5p and miR-125a-5p to promote the tumorigenesis and progression of spinal chordoma. LINC00525 can be a novel molecular target in spinal chordoma.
- Subjects :
- Male
musculoskeletal diseases
0301 basic medicine
Epithelial-Mesenchymal Transition
Biophysics
Down-Regulation
Mice, Nude
Apoptosis
medicine.disease_cause
Biochemistry
03 medical and health sciences
0302 clinical medicine
Cell Movement
Cell Line, Tumor
Chordoma
medicine
Animals
Humans
Neoplasm Invasiveness
Gene Silencing
Neoplasm Metastasis
Spinal Chordoma
Molecular Biology
Cell Proliferation
Mice, Inbred BALB C
Gene knockdown
Spinal Neoplasms
Base Sequence
Cell growth
Chemistry
RNA
Oncogenes
Cell Biology
Long non-coding RNA
Up-Regulation
Gene Expression Regulation, Neoplastic
mir-31
MicroRNAs
030104 developmental biology
030220 oncology & carcinogenesis
Cancer research
RNA, Long Noncoding
Carcinogenesis
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 536
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....5064b1dea6e3b4f1e0357e2a0562ce16