Growth, survival, nutritional value and phytochemical, and antioxidant state of Litopenaeus vannamei shrimp fed with premix extract of brown Sargassum ilicifolium, Nizimuddinia zanardini, Cystoseira indica, and Padina australis macroalgae

The effect of including the premix extract of the brown Sargassum ilicifolium, Nizimuddinia zanardini, Cystoseira indica and Padina australis (MPE) macroalgae in the diet on the growth performance, survival, nutritional (chemical, amino acid and fatty acid compound) and phytochemical (sterol, phenol and flavonoid),and antioxidant state (superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and glutathione peroxidase (GPX)) of whiteleg shrimp (Litopenaeus vannamei) was investigated in this study, where in 1200 post larvae with an average weight of 57.530.47 mg were distributed randomly by categorizing 100 pieces into 4 groups (three tank iterations per group). The control group was only fed with the concentrated feed (MPE0), while the other groups were fed with a basic diet that consisted of MPE5 (MPE5), 10 (MPE10) and 15 (MPE15) g kg− 1 of feed for 8 weeks. According to the results, the highest FW, SGR, WG and PER were recorded for the MPE15 group. The lowest FCR was observed in the group fed with MPE15 (P 10 and MPE15 (P 15. The results are generally indicative of the positive effect of the premix aqueous extract of the brown S. ilicifolium, N. zanardini, C. indica and P.australis macroalgae at the 15 g kg− 1 of MPE feed level on the growth, nutritional value and phytochemical, and antioxidant status of L. vannamei.