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Preferential expression of the Drosophila rutabaga gene in mushroom bodies, neural centers for learning in insects
- Source :
- Neuron. 9(4)
- Publication Year :
- 1992
-
Abstract
- Seven lines were isolated with P element insertions in the cytogenetic vicinity of the learning and memory gene, rutabaga, from an enhancer detector screen designed to mark genes preferentially expressed in mushroom bodies. Six of these lines performed poorly in learning and memory tests, and several failed to complement an existing rutabaga allele. Molecular cloning revealed that the P elements were inserted in the putative promoter of the rutabaga gene. RNA in situ hybridization and immunohistochemistry demonstrated that the expression of the rutabaga gene, which encodes a Ca2+/calmodulin-responsive adenylyl cyclase, is markedly elevated in the mushroom bodies of normal flies and that the insertion elements compromised its expression in the new rutabaga mutants. The reisolation of a known learning and memory gene, but with a heretofore unknown expression pattern, strongly supports the postulate that mushroom bodies are principal sites mediating olfactory learning and memory.
- Subjects :
- Kenyon cell
Mutant
Molecular Sequence Data
Gene Expression
Biology
Nervous System
P element
Memory
Drosophilidae
Gene expression
Animals
Learning
Nervous System Physiological Phenomena
Cloning, Molecular
Enhancer
Promoter Regions, Genetic
Gene
Alleles
In Situ Hybridization
Genetics
Base Sequence
General Neuroscience
biology.organism_classification
beta-Galactosidase
Immunohistochemistry
Enhancer Elements, Genetic
Mushroom bodies
DNA Transposable Elements
RNA
Drosophila
Subjects
Details
- ISSN :
- 08966273
- Volume :
- 9
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Neuron
- Accession number :
- edsair.doi.dedup.....5046e90536f5e58609d4ba356f46a2cf