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DNA methylation Profiles in Primary Cutaneous Melanomas are Associated with Clinically Significant Pathologic Features
- Publication Year :
- 2014
-
Abstract
- Summary DNA methylation studies have elucidated a methylation signature distinguishing primary melanomas from benign nevi and provided new insights about genes that may be important in melanoma development. However, it is unclear whether methylation differences among primary melanomas are related to tumor pathologic features with known clinical significance. We utilized the Illumina GoldenGate Cancer Panel array to investigate the methylation profiles of 47 primary cutaneous melanomas. Arraywide methylation patterns revealed a positive association of methylation with Breslow thickness and mutated BRAF, a negative association with mitotic rate, and a weak association with ulceration. Hierarchical clustering on CpG sites exhibiting the most variable methylation (n = 235) divided the melanoma samples into three clusters, including a highly methylated cluster that was positively associated with Breslow thickness and an intermediately methylated cluster associated with Breslow thickness and mitotic rate. Our findings provide support for the existence of methylation-defined subsets in melanomas with increased methylation associated with Breslow thickness.
- Subjects :
- Adult
Male
Skin Neoplasms
Molecular Sequence Data
Dermatology
Biology
Bioinformatics
General Biochemistry, Genetics and Molecular Biology
Article
Breslow Thickness
medicine
Cluster Analysis
Humans
Clinical significance
Promoter Regions, Genetic
neoplasms
Melanoma
Aged
Demography
CpG Island Methylator Phenotype
Cancer
Reproducibility of Results
Methylation
DNA Methylation
Middle Aged
medicine.disease
Phenotype
Oncology
CpG site
Genetic Loci
DNA methylation
Cancer research
CpG Islands
Female
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....50440fbe670768b18204cf2c15b0787d