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Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters
- Source :
- Nutrients; Volume 14; Issue 19; Pages: 4177
- Publication Year :
- 2022
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2022.
-
Abstract
- Background: The hypothalamic paraventricular nucleus (PVN) is an important nucleus in the brain that plays a key role in regulating sympathetic nerve activity (SNA) and blood pressure. Silent mating-type information regulation 2 homolog-1 (sirtuin1, SIRT1) not only protects cardiovascular function but also reduces inflammation and oxidative stress in the periphery. However, its role in the central regulation of hypertension remains unknown. It is hypothesized that SIRT1 activation by resveratrol may reduce SNA and lower blood pressure through the regulation of intracellular reactive oxygen species (ROS) and neurotransmitters in the PVN. Methods: The two-kidney one-clip (2K1C) method was used to induce renovascular hypertension in male Sprague-Dawley rats. Then, bilaterally injections of vehicle (artificial cerebrospinal fluid, aCSF, 0.4 μL) or resveratrol (a SIRT1 agonist, 160 μmol/L, 0.4 μL) into rat PVN were performed for four weeks. Results: PVN SIRT1 expression was lower in the hypertension group than the sham surgery (SHAM) group. Activated SIRT1 within the PVN lowered systolic blood pressure and plasma norepinephrine (NE) levels. It was found that PVN of 2K1C animals injected with resveratrol exhibited increased expression of SIRT1, copper-zinc superoxide dismutase (SOD1), and glutamic acid decarboxylase (GAD67), as well as decreased activity of nuclear factor-kappa B (NF-κB) p65 and NAD(P)H oxidase (NOX), particularly NOX4. Treatment with resveratrol also decreased expression of ROS and tyrosine hydroxylase (TH). Conclusion: Resveratrol within the PVN attenuates hypertension via the SIRT1/NF-κB pathway to decrease ROS and restore the balance of excitatory and inhibitory neurotransmitters.
- Subjects :
- Male
Neurotransmitter Agents
Sympathetic Nervous System
Nutrition and Dietetics
Tyrosine 3-Monooxygenase
Glutamate Decarboxylase
NF-kappa B
NADPH Oxidases
resveratrol
high blood pressure
PVN
SIRT1
ROS
excitatory and inhibitory neurotransmitters
Rats
Rats, Sprague-Dawley
Norepinephrine
Zinc
Superoxide Dismutase-1
Sirtuin 1
Resveratrol
Hypertension
Animals
Reactive Oxygen Species
Copper
Paraventricular Hypothalamic Nucleus
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 20726643
- Database :
- OpenAIRE
- Journal :
- Nutrients; Volume 14; Issue 19; Pages: 4177
- Accession number :
- edsair.doi.dedup.....502b92b587064acc3ac1c8930839459a
- Full Text :
- https://doi.org/10.3390/nu14194177