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Transport stress induces apoptosis in rat myocardial tissue via activation of the mitogen-activated protein kinase signaling pathways
- Source :
- Heart and Vessels. 31:212-221
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- The present study aimed to elucidate the mechanism of myocardial damage induced by simulated transport stress. Sprague-Dawley rats were subjected to 35 °C and 60 rpm (0.1×g rcf) on a constant temperature shaker. The blood samples were prepared for detection of epinephrine (E), norepinephrine (NE), atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and serum cardiac troponin T (cTNT); myocardium samples were prepared for morphological examination and signaling protein quantitative. The result showed that plasma norepinephrine (NE) and epinephrine (E) concentrations increased in all stressed groups (P < 0.01). Levels of serum cardiac troponin T (cTNT) were elevated in both the S2d (P < 0.05) and S3d groups (P < 0.01). The concentration of plasma BNP was increased significantly in S3d group (P < 0.05); the difference in ANP was not remarkable. Morphological observation demonstrated obvious microstructure and ultrastructure damage after simulated transport stress. There was also a significant increase in the number of TUNEL-positive cardiomyocytes in stressed hearts. Western blot analysis found that the mitogen-activated protein kinase (MAPK) pathways were activated by strengthening phosphorylation of ASK-1, JNK, P38 and ERK in rat myocardial tissue after simulated transport stress (P < 0.05, P < 0.01). In addition, the ratio of pro-apoptotic Bax and anti-apoptotic Bcl-2 proteins was increased in stressed rats (P < 0.01), and the amount of cleaved-caspase3 increased in all stressed rats (P < 0.01). The expression of cleaved-caspase9 protein was also elevated in S2d and S3d groups (P < 0.01). Consequently simulated transport stress induced obvious myocardial damage, which may be attributed to the activation of caspase 9-mediated mitochondrial apoptotic pathway and MAPK pathways.
- Subjects :
- Male
0301 basic medicine
MAPK/ERK pathway
medicine.medical_specialty
Hot Temperature
Time Factors
Epinephrine
p38 mitogen-activated protein kinases
Apoptosis
Transportation
030204 cardiovascular system & hematology
Rats, Sprague-Dawley
Norepinephrine
03 medical and health sciences
0302 clinical medicine
Troponin T
Troponin complex
Atrial natriuretic peptide
Western blot
Stress, Physiological
Internal medicine
Natriuretic Peptide, Brain
medicine
Animals
Phosphorylation
Protein kinase A
biology
medicine.diagnostic_test
business.industry
Myocardium
Brain natriuretic peptide
Enzyme Activation
030104 developmental biology
Endocrinology
Mitogen-activated protein kinase
biology.protein
Mitogen-Activated Protein Kinases
Apoptosis Regulatory Proteins
Cardiology and Cardiovascular Medicine
business
Atrial Natriuretic Factor
Biomarkers
Signal Transduction
Subjects
Details
- ISSN :
- 16152573 and 09108327
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Heart and Vessels
- Accession number :
- edsair.doi.dedup.....50276299a5fa5954c198802c237a38f4
- Full Text :
- https://doi.org/10.1007/s00380-014-0607-3