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Tissue alarmins and adaptive cytokine induce dynamic and distinct transcriptional responses in tissue-resident intraepithelial cytotoxic T lymphocytes
- Source :
- Journal of Autoimmunity, Journal of Autoimmunity, 108:102422. ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD, Journal of autoimmunity
- Publication Year :
- 2019
-
Abstract
- The respective effects of tissue alarmins interleukin (IL)-15 and interferon beta (IFNβ), and IL-21 produced by T cells on the reprogramming of cytotoxic T lymphocytes (CTLs) that cause tissue destruction in celiac disease is poorly understood. Transcriptomic and epigenetic profiling of primary intestinal CTLs showed massive and distinct temporal transcriptional changes in response to tissue alarmins, while the impact of IL-21 was limited. Only anti-viral pathways were induced in response to all the three stimuli, albeit with differences in dynamics and strength. Moreover, changes in gene expression were primarily independent of changes in H3K27ac, suggesting that other regulatory mechanisms drive the robust transcriptional response. Finally, we found that IL-15/IFNβ/IL-21 transcriptional signatures could be linked to transcriptional alterations in risk loci for complex immune diseases. Together these results provide new insights into molecular mechanisms that fuel the activation of CTLs under conditions that emulate the inflammatory environment in patients with autoimmune diseases.<br />Highlights • We report the epigenetic and transcriptomic response of IE-CTLs to tissue-derived alarmins and adaptive cytokine. • IL-15 and IFNβ induce massive and distinct transcriptional changes in IE-CTLs, while the impact of IL-21 was limited. • Genes associated with immune-mediated intestinal diseases are enriched in genes responding to IL-15, IFNβ and IL-21 in IE-CTLs.
- Subjects :
- 0301 basic medicine
medicine.medical_treatment
Autoimmunity
Intraepithelial lymphocytes
DISEASE
Transcriptome
Tissue-resident lymphocytes
0302 clinical medicine
Autoimmune disease
IL-21
Immunology and Allergy
Cytotoxic T cell
Alarmins
Intestinal Mucosa
Promoter Regions, Genetic
Interleukin-15
ARCHITECTURE
Interleukin
ASSOCIATION
3. Good health
Cell biology
Cytokine
IL-15
Interleukin 15
Cytokines
EXPRESSION
SUSCEPTIBILITY LOCI
Immunology
EFFECTOR CTL
GENETIC RISK
IMMUNITY
Biology
IFN beta
Article
Tissue-resident lymphocyte
03 medical and health sciences
medicine
Humans
Epigenetics
030203 arthritis & rheumatology
Cytotoxic T lymphocyte
Intraepithelial lymphocyte
IDENTIFICATION
Gene Expression Profiling
medicine.disease
Inflammatory Bowel Diseases
Cytotoxic T lymphocytes
Celiac Disease
030104 developmental biology
Gene Expression Regulation
CELLS
IFNβ
T-Lymphocytes, Cytotoxic
Subjects
Details
- ISSN :
- 10959157 and 08968411
- Volume :
- 108
- Database :
- OpenAIRE
- Journal :
- Journal of autoimmunity
- Accession number :
- edsair.doi.dedup.....4fec04b3696dc883712b72d6fe1ecd08