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Discovery of aphid-transmitted Rice tiller inhibition virus from native plants through metagenomic sequencing
- Source :
- PLoS pathogens, vol 19, iss 3
- Publication Year :
- 2023
- Publisher :
- eScholarship, University of California, 2023.
-
Abstract
- A major threat to rice production is the disease epidemics caused by insect-borne viruses that emerge and re-emerge with undefined origins. It is well known that some human viruses have zoonotic origins from wild animals. However, it remains unknown whether native plants host uncharacterized endemic viruses with spillover potential to rice (Oryza sativa) as emerging pathogens. Here, we discovered rice tiller inhibition virus (RTIV), a novel RNA virus species, from colonies of Asian wild rice (O. rufipogon) in a genetic reserve by metagenomic sequencing. We identified the specific aphid vector that is able to transmit RTIV and found that RTIV would cause low-tillering disease in rice cultivar after transmission. We further demonstrated that an infectious molecular clone of RTIV initiated systemic infection and causes low-tillering disease in an elite rice variety after Agrobacterium-mediated inoculation or stable plant transformation, and RTIV can also be transmitted from transgenic rice plant through its aphid vector to cause disease. Finally, global transcriptome analysis indicated that RTIV may disturb defense and tillering pathway to cause low tillering disease in rice cultivar. Thus, our results show that new rice viral pathogens can emerge from native habitats, and RTIV, a rare aphid-transmitted rice viral pathogen from native wild rice, can threaten the production of rice cultivar after spillover.
- Subjects :
- Gene Expression Profiling
Immunology
Oryza
Genetically Modified
Plants
Microbiology
Vector-Borne Diseases
Emerging Infectious Diseases
Infectious Diseases
Good Health and Well Being
Medical Microbiology
Aphids
Virology
Viruses
Genetics
Animals
Humans
2.1 Biological and endogenous factors
2.2 Factors relating to the physical environment
Parasitology
Aetiology
Infection
Molecular Biology
Plant Diseases
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- PLoS pathogens, vol 19, iss 3
- Accession number :
- edsair.doi.dedup.....4f68c5bd30088a45ad71ada2a5a2cbc3