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A High Throughput Screening HPLC-FLD Method for Paralytic Shellfish Toxins (PSTs) Enabling Effective Official Control
- Source :
- Molecules; Volume 27; Issue 15; Pages: 4702
- Publication Year :
- 2022
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2022.
-
Abstract
- Paralytic Shellfish Toxins (PSTs) are marine biotoxins, primarily produced by dinoflagellates of the genera Gymnodinium spp., Alexandrium spp. They can accumulate in shellfish and, through the food chain, be assimilated by humans, giving rise to Paralytic Shellfish Poisoning. The maximum permitted level for PSTs in bivalves is 800 μg STX·2HCl eqv/kg (Reg. EC N° 853/2004). Until recently, the reference analytical method was the Mouse Bioassay, but Reg. EU N° 1709/2021 entered into force on 13 October 2021 and identified in the Standard EN14526:2017 or in any other internationally recognized validated method not entailing the use of live animals as official methods. Then the official control laboratories had urgently to fulfill the new requests, face out the Mouse Bioassay and implement instrumental analytical methods. The “EURLMB SOP for the analysis of PSTs by pre-column HPLC-FLD according to OMA AOAC 2005.06” also introduced a simplified semiquantitative approach to discriminate samples above and below the regulatory limit. The aim of the present paper is to present a new presence/absence test with a cut-off at 600 μg STX·2HCl eqv/kg enabling the fast discrimination of samples with very low PSTs levels from those to be submitted to the full quantitative confirmatory EN14526:2017 method. The method was implemented, avoiding the use of a large number of certified reference standards and long quantification procedures, resulting in an efficient, economical screening instrument available for official control laboratories. The protocol was fully validated, obtaining good performances in terms of repeatability (
- Subjects :
- paralytic shellfish toxins
HPLC-FLD
official control
screening method
marine biotoxins
gastropods
Organic Chemistry
Pharmaceutical Science
Analytical Chemistry
Bivalvia
High-Throughput Screening Assays
Mice
Chemistry (miscellaneous)
Drug Discovery
Dinoflagellida
Molecular Medicine
Animals
Humans
Shellfish Poisoning
Marine Toxins
Physical and Theoretical Chemistry
Chromatography, High Pressure Liquid
Shellfish
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules; Volume 27; Issue 15; Pages: 4702
- Accession number :
- edsair.doi.dedup.....4f517ff1037e47db07bd0bf3d091bb9d
- Full Text :
- https://doi.org/10.3390/molecules27154702