Additional file 3 of A novel c.64G > T (p.G22C) NR5A1 variant in a Chinese adolescent with 46,XY disorders of sex development: a case report

Additional file 3: Supplementary Fig 3. The raw figures of gels (EMSA) and the blots (Western blot) (A) the bands of the internal reference (β-actin). From left to right, these bands are non-transfected (Control, undeveloped band), transfected with an empty vector (Vector), Myc-tagged WT (WT), and c.64G>T (p. G22C) NR5A1-Mut (Mut) vectors; (B) NR5A1 expression in 293T cells according to western blot analysis. (same grouping as A); (C) ESMAs results showing altered DNA binding by the NR5A1 mutant (same group as A).