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Large field-of-view phase and fluorescence mesoscope with microscopic resolution
- Source :
- Journal of Biomedical Optics, Journal of Biomedical Optics, 2019, 24 (3), pp.036501. ⟨10.1117/1.JBO.24.3.036501⟩, Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2019, 24 (3), pp.036501. ⟨10.1117/1.JBO.24.3.036501⟩
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- International audience; Phase and fluorescence are complementary contrasts that are commonly used in biology. However, the coupling of these two modalities is traditionally limited to high magnification and complex imaging systems. For statistical studies of biological populations, a large field-of-view is required. We describe a 30 mm(2) field-of-view dual-modality mesoscope with a 4-mu m resolution. The potential of the system to address biological questions is illustrated on white blood cell numeration in whole blood and multiwavelength imaging of the human osteosarcoma (U2-OS) cells.
- Subjects :
- Paper
Cytological Techniques
Biomedical Engineering
Holography
Phase (waves)
01 natural sciences
in-line holography Paper 180667R
law.invention
010309 optics
Biomaterials
[SPI]Engineering Sciences [physics]
law
Cell Line, Tumor
0103 physical sciences
Microscopy
Image Processing, Computer-Assisted
Humans
phase
Image resolution
Large field of view
High magnification
Resolution (electron density)
Equipment Design
Fluorescence
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Microscopy, Fluorescence
microscopy
fluorescence
Biological system
in-line holography
Algorithms
Subjects
Details
- Language :
- English
- ISSN :
- 10833668
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics, Journal of Biomedical Optics, 2019, 24 (3), pp.036501. ⟨10.1117/1.JBO.24.3.036501⟩, Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2019, 24 (3), pp.036501. ⟨10.1117/1.JBO.24.3.036501⟩
- Accession number :
- edsair.doi.dedup.....4f20d94b81b4ff37fc1b29f5750c73c3