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A Fluidic Device for Immunomagnetic Separation of Foodborne Bacteria Using Self-Assembled Magnetic Nanoparticle Chains
- Source :
- Micromachines, Volume 9, Issue 12, Micromachines, Vol 9, Iss 12, p 624 (2018)
- Publication Year :
- 2018
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2018.
-
Abstract
- Immunomagnetic separation has been widely used for the separation and concentration of foodborne pathogens from complex food samples, however it can only handle a small volume of samples. In this paper, we presented a novel fluidic device for the specific and efficient separation and concentration of salmonella typhimurium using self-assembled magnetic nanoparticle chains. The laminated sawtooth-shaped iron foils were first mounted in the 3D-printed matrix and magnetized by a strong magnet to generate dot-array high gradient magnetic fields in the fluidic channel, which was simulated using COMSOL (5.3a, Burlington, MA, USA). Then, magnetic nanoparticles with a diameter of 150 nm, which were modified with the anti-salmonella polyclonal antibodies, were injected into the channel, and the magnetic nanoparticle chains were vertically formed at the dots and verified using a fluorescence inverted microscope. Finally, the bacterial sample was continuous-flow injected, and the target bacteria could be captured by the antibodies on the chains, followed by gold standard culture plating to determine the amount of the target bacteria. Under the optimal conditions, the target bacteria could be separated with a separation efficiency of 80% in 45 min. This fluidic device could be further improved using thinner sawtooth-shaped iron foils and stronger magnets to obtain a better dot-array magnetic field with larger magnetic intensity and denser dot distribution, and has the potential to be integrated with the existing biological assays for rapid and sensitive detection of foodborne bacteria.
- Subjects :
- Materials science
lcsh:Mechanical engineering and machinery
salmonella
Nanoparticle
02 engineering and technology
Immunomagnetic separation
01 natural sciences
Article
Matrix (chemical analysis)
lcsh:TJ1-1570
Fluidics
Electrical and Electronic Engineering
Mechanical Engineering
010401 analytical chemistry
Inverted microscope
021001 nanoscience & nanotechnology
equipment and supplies
fluidic chip
immunomagnetic separation
0104 chemical sciences
Magnetic field
magnetic nanoparticle chains
Chemical engineering
Control and Systems Engineering
Magnet
Magnetic nanoparticles
0210 nano-technology
human activities
Subjects
Details
- Language :
- English
- ISSN :
- 2072666X
- Database :
- OpenAIRE
- Journal :
- Micromachines
- Accession number :
- edsair.doi.dedup.....4f1588a2001db65def8c0511d00ef674
- Full Text :
- https://doi.org/10.3390/mi9120624