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Glucocorticoids decrease Treg cell numbers in lungs of allergic mice
- Source :
- European Journal of Pharmacology. 747:52-58
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Glucocorticoids have been the hallmark anti-inflammatory drug used to treat asthma. It has been shown that glucocorticoids ameliorate asthma by increasing numbers and activity of Tregs, in contrast recent data show that glucocorticoid might have an opposite effect on Treg cells from normal mice. Since Tregs are target cells that act on the resolution of asthma, the aim of this study was to elucidate the effect of glucocorticoid treatment on lung Tregs in mouse models of asthma. Allergen challenged mice were treated with either oral dexamethasone or nebulized budesonide. Broncoalveolar lavage and airway hyperresponsiveness were evaluated after allergenic challenge. Lung, thymic and lymph node cells were phenotyped on Treg through flow cytometry. Lung cytokine secretion was detected by ELISA. Although dexamethasone inhibited airway inflammation and hyperresponsiveness, improving resolution, we have found that both dexamethasone and budesonide induce a reduction of Treg numbers on lungs and lymphoid organs of allergen challenged mice. The reduction of lung Treg levels was independent of mice strain or type of allergen challenge. Our study also indicates that both glucocorticoids do not increase Treg activity through production of IL-10. Glucocorticoid systemic or localized treatment induced thymic atrophy. Taken together, our results demonstrate that glucocorticoids decrease Treg numbers and activity in different asthma mouse models, probably by reducing thymic production of T cells. Therefore, it is possible that glucocorticoids do not have beneficial effects on lung populations of Treg cells from asthmatic patients.
- Subjects :
- Male
Budesonide
Ovalbumin
Cell Count
Thymus Gland
T-Lymphocytes, Regulatory
Dexamethasone
Flow cytometry
Mice
medicine
Animals
Glucocorticoids
Lung
Asthma
Pharmacology
medicine.diagnostic_test
business.industry
Pyroglyphidae
Pneumonia
respiratory system
medicine.disease
Interleukin-10
respiratory tract diseases
Lymphatic system
medicine.anatomical_structure
Immunology
Cytokine secretion
business
Glucocorticoid
medicine.drug
Subjects
Details
- ISSN :
- 00142999
- Volume :
- 747
- Database :
- OpenAIRE
- Journal :
- European Journal of Pharmacology
- Accession number :
- edsair.doi.dedup.....4f1445a946b505f89d954c7d13482b59
- Full Text :
- https://doi.org/10.1016/j.ejphar.2014.11.034