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A Chromatin-remodeling Protein Is a Component of Fission Yeast Mediator*
- Publication Year :
- 2010
- Publisher :
- American Society for Biochemistry and Molecular Biology, 2010.
-
Abstract
- The multiprotein Mediator complex is an important regulator of RNA polymerase II-dependent genes in eukaryotic cells. In contrast to the situation in many other eukaryotes, the conserved Med15 protein is not a stable component of Mediator isolated from fission yeast. We here demonstrate that Med15 exists in a protein complex together with Hrp1, a CHD1 ATP-dependent chromatin-remodeling protein. The Med15-Hrp1 subcomplex is not a component of the core Mediator complex but can interact with the L-Mediator conformation. Deletion of med15(+) and hrp1(+) causes very similar effects on global steady-state levels of mRNA, and genome-wide analyses demonstrate that Med15 associates with a distinct subset of Hrp1-bound gene promoters. Our findings therefore indicate that Mediator may directly influence histone density at regulated promoters.
- Subjects :
- RNA polymerase II
Biochemistry
Chromatin remodeling
MED1
Histones
Core mediator complex
Mediator
Schizosaccharomyces
Gene Regulation
RNA, Messenger
Promoter Regions, Genetic
Molecular Biology
Genetics
Messenger RNA
Mediator Complex
biology
DNA Helicases
RNA, Fungal
Cell Biology
Chromatin
Cell biology
Histone
biology.protein
Trans-Activators
Schizosaccharomyces pombe Proteins
Genome, Fungal
Gene Deletion
Genome-Wide Association Study
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....4f109c5275207755841f4bae2d8e3691