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RNAi-mediated silencing of CD147 inhibits tumor cell proliferation, invasion and increases chemosensitivity to cisplatin in SGC7901 cells in vitro
- Source :
- Journal of Experimental & Clinical Cancer Research : CR, Journal of Experimental & Clinical Cancer Research, Vol 29, Iss 1, p 61 (2010)
- Publication Year :
- 2010
-
Abstract
- Background CD147 is a widely distributed cell surface glycoprotein that belongs to the Ig superfamily. CD147 has been implicated in numerous physiological and pathological activities. Enriched on the surface of many tumor cells, CD147 promotes tumor growth, invasion, metastasis and angiogenesis and confers resistance to some chemotherapeutic drugs. In this study, we investigated the possible role of CD147 in the progression of gastric cancer. Methods Short hairpin RNA (shRNA) expressing vectors targeting CD147 were constructed and transfected into human gastric cancer cells SGC7901 and CD147 expression was monitored by quantitative realtime RT-PCR and Western blot. Cell proliferation, the activities of MMP-2 and MMP-9, the invasive potential and chemosensitivity to cisplatin of SGC7901 cells were determined by MTT, gelatin zymography, Transwell invasion assay and MTT, respectively. Results Down-regulation of CD147 by RNAi approach led to decreased cell proliferation, MMP-2 and MMP-9 activities and invasive potential of SGC7901 cells as well as increased chemosensitivity to cisplatin. Conclusion CD147 involves in proliferation, invasion and chemosensitivity of human gastric cancer cell line SGC7901, indicating that CD147 may be a promising therapeutic target for gastric cancer.
- Subjects :
- Cancer Research
Angiogenesis
Cell
Blotting, Western
Down-Regulation
Antineoplastic Agents
Apoptosis
Biology
lcsh:RC254-282
Metastasis
Small hairpin RNA
Cell Movement
Stomach Neoplasms
medicine
Cell Adhesion
Tumor Cells, Cultured
Humans
RNA, Messenger
RNA, Small Interfering
Cell adhesion
Cell Proliferation
Cell growth
Reverse Transcriptase Polymerase Chain Reaction
Research
Cancer
medicine.disease
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Molecular biology
Gene Expression Regulation, Neoplastic
medicine.anatomical_structure
Oncology
Matrix Metalloproteinase 9
Cancer cell
Cancer research
Basigin
Matrix Metalloproteinase 2
Cisplatin
Subjects
Details
- ISSN :
- 17569966
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Journal of experimentalclinical cancer research : CR
- Accession number :
- edsair.doi.dedup.....4ef3f192af8757752c1545731d307a05