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Elevated free fatty acids and impaired adiponectin bioactivity contribute to reduced SOD2 protein in monocytes of type 2 diabetes patients
- Source :
- Experimental and Molecular Pathology. 90:101-106
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- Type 2 diabetes (T2D) is characterized by increased oxidative stress contributing to the development of cardiovascular disease (CVD). Monocytes are critically important in the pathogenesis of CVD and antioxidant enzymes like superoxide dismutase (SOD2) protect these cells from excessive reactive oxygen species (ROS). Adiponectin is an adipocyte-derived protein with atheroprotective function and the effect of adiponectin on monocyte SOD2 was analyzed herein. Adiponectin upregulated SOD2 mRNA and dose- and time-dependently induced SOD2 protein in primary human monocytes. Elevated systemic free fatty acids (FFA) are commonly found in T2D patients and palmitic acid as well as oleic acid reduced monocyte SOD2 protein. Adiponectin mediated upregulation of SOD2, however, was not affected by FFA incubation. SOD2 protein was reduced in T2D monocytes compared to monocytes of age- and body mass index-matched healthy controls. Adiponectin still induced SOD2 in T2D monocytes but efficiency tended to be reduced. In summary this study indicates that elevated systemic free fatty acids and impaired adiponectin activity contribute to reduced SOD2 and most likely increased oxidative stress in T2D monocytes.
- Subjects :
- Adult
Male
medicine.medical_specialty
Antioxidant
medicine.medical_treatment
Clinical Biochemistry
SOD2
Fatty Acids, Nonesterified
Biology
medicine.disease_cause
Monocytes
Body Mass Index
Pathology and Forensic Medicine
Superoxide dismutase
Downregulation and upregulation
Internal medicine
Adipocytes
medicine
Humans
skin and connective tissue diseases
Molecular Biology
Cells, Cultured
Aged
chemistry.chemical_classification
Reactive oxygen species
Dose-Response Relationship, Drug
Adiponectin
Superoxide Dismutase
Monocyte
nutritional and metabolic diseases
Middle Aged
Recombinant Proteins
Endocrinology
medicine.anatomical_structure
Diabetes Mellitus, Type 2
chemistry
Case-Control Studies
cardiovascular system
biology.protein
Oxidative stress
Subjects
Details
- ISSN :
- 00144800
- Volume :
- 90
- Database :
- OpenAIRE
- Journal :
- Experimental and Molecular Pathology
- Accession number :
- edsair.doi.dedup.....4ec77cdcfd9e9d500f2bd053feb06426