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Expression and function of α1-adrenoceptor subtypes in the porcine renal artery
- Source :
- European Journal of Pharmacology. 341:95-103
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- We investigated the expression and function of alpha1-adrenoceptor subtypes in the porcine renal artery. Reverse transcription polymerase chain reaction (RT-PCR) and nucleotide sequencing indicated that the mRNAs for alpha1a- and alpha1b-adrenoceptors were expressed in the porcine renal artery. Chloroethylclonidine, an alpha1B- and alpha1D-adrenoceptor antagonist, partially inhibited the phenylephrine-induced contraction, while 3 nM BMY 7378 (8-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]-8-azaspiro[4.5]dec ane-7,9-dione dihydrochloride), an alpha1D-adrenoceptor antagonist, had no effect. In contrast, 5-methylurapidil, an alpha1A-adrenoceptor antagonist, induced a rightward shift of the phenylephrine concentration-response curve. The simultaneous measurement of cytosolic Ca2+ concentration ([Ca2+]i) and tension revealed that chloroethylclonidine pretreatment abolished the phenylephrine-induced increases in [Ca2+]i and tension in the Ca2+-free solution. The application of 5-methylurapidil (3 nM) to the chloroethylclonidine-pretreated strips completely inhibited the 3 microM phenylephrine-induced [Ca2+]i and tension increase in normal PSS. We concluded that both alpha1A- and alpha1B-adrenoceptors mediate the phenylephrine-induced contraction of the porcine renal artery accompanied by an increase in [Ca2+]i, and that alpha1A-adrenoceptors cause Ca2+ influx whereas alpha1B-adrenoceptors mainly mediate Ca2+ release.
- Subjects :
- Male
medicine.medical_specialty
Contraction (grammar)
Swine
medicine.medical_treatment
Molecular Sequence Data
Gene Expression
Biology
Clonidine
Phenylephrine
chemistry.chemical_compound
Renal Artery
Chloroethylclonidine
Receptors, Adrenergic, alpha-1
Internal medicine
medicine.artery
medicine
Animals
Amino Acid Sequence
RNA, Messenger
Renal artery
Antihypotensive agent
Adrenergic alpha-Antagonists
Pharmacology
Base Sequence
Antagonist
Endocrinology
chemistry
Adrenergic alpha-1 Receptor Antagonists
Calcium
Female
medicine.symptom
Adrenergic alpha-Agonists
Vasoconstriction
Muscle Contraction
medicine.drug
Muscle contraction
Subjects
Details
- ISSN :
- 00142999
- Volume :
- 341
- Database :
- OpenAIRE
- Journal :
- European Journal of Pharmacology
- Accession number :
- edsair.doi.dedup.....4ebc8c4d4a9184bf6e5a896b34e334a3