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Polyclonal spread of multiple genotypes of Mycoplasma pneumoniae in semi-closed settings in Yamagata, Japan
- Source :
- Journal of Medical Microbiology. 68:785-790
- Publication Year :
- 2019
- Publisher :
- Microbiology Society, 2019.
-
Abstract
- Purpose. To clarify the spread of Mycoplasma pneumoniae infections in semi-closed settings such as schools and family homes using molecular typing methods. Methodology. We retrospectively searched for school- and family-based clusters of M. pneumoniae infections based on information regarding patients from whom M. pneumoniae strains had been isolated between 2011 and 2013 in Yamagata, Japan. The molecular typing profile, including the P1 type and the four-locus (Mpn13, 14, 15 and 16) multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) type, was obtained from our previous study. Results. We identified 11 school-based clusters involving 71 patients and 16 family-based clusters involving 38 patients, including 14 duplications between these types of clusters. A total of 95 M. pneumoniae strains isolated from those patients were divided into 4 genotypes: 33 strains of type 4-5-7-2, 1; 31 of type 4-5-7-3, 1; 24 of type 3-5-6-2, 2c; and 7 of type 3-5-6-2, 2a. Of the 11 school-based clusters, 6 clusters (54.5%) consisted of multiple genotypes, and the remaining 5 clusters consisted of a single genotype. Moreover, the presence of multiple genotypes was identified in three classrooms of a school. On the other hand, in 14 (87.5%) of the 16 family-based clusters, the genotypes of the M. pneumoniae strains isolated from each family member were identical. Conclusion. The spread of M. pneumoniae infection in schools is likely polyclonal, since M. pneumoniae strains are brought into schools from various sites, such as family homes, which are important sites of disease transmission.
- Subjects :
- DNA, Bacterial
0301 basic medicine
Microbiology (medical)
Mycoplasma pneumoniae
Genotype
030106 microbiology
Microbial Sensitivity Tests
Minisatellite Repeats
Biology
Multiple Loci VNTR Analysis
medicine.disease_cause
Microbiology
03 medical and health sciences
Molecular typing
Japan
Pneumonia, Mycoplasma
medicine
Humans
Child
Retrospective Studies
Family Characteristics
Schools
General Medicine
Mycoplasma pneumoniae Infections
Virology
Molecular Typing
Family member
030104 developmental biology
Polyclonal antibodies
biology.protein
Disease transmission
Subjects
Details
- ISSN :
- 14735644 and 00222615
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Microbiology
- Accession number :
- edsair.doi.dedup.....4ea45d172c5f041481c2948ad3cb440f