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Activity-dependent labeling of oxygenase enzymes in a trichloroethene-contaminated groundwater site
- Source :
- Environmental pollution (Barking, Essex : 1987). 153(1)
- Publication Year :
- 2006
-
Abstract
- A variety of naturally occurring bacteria produce enzymes that cometabolically degrade trichloroethene (TCE), including organisms with aerobic oxygenases. Groundwater contaminated with TCE was collected from the aerobic region of the Test Area North site of the Idaho National Laboratory. Samples were evaluated with enzyme activity probes, and resulted in measurable detection of toluene oxygenase activity (6-79% of the total microbial cells). Wells from both inside and outside contaminated plume showed activity. Toluene oxygenase-specific PCR primers determined that toluene-degrading genes were present in all groundwater samples evaluated. In addition, bacterial isolates were obtained and possessed toluene oxygenase enzymes, demonstrated activity, and were dominated by the phylotype Pseudomonas. This study demonstrated, through the use of enzymatic probes and oxygenase gene identification, that indigenous microorganisms at a contaminated site were cometabolically active. Documentation such as this can be used to substantiate observations of natural attenuation of TCE-contaminated groundwater plumes.
- Subjects :
- Oxygenase
Health, Toxicology and Mutagenesis
Idaho
Mineralogy
Cometabolism
Fresh Water
Toxicology
Polymerase Chain Reaction
chemistry.chemical_compound
Pseudomonas
Water pollution
DNA Primers
Phylotype
biology
Bacteria
Ecology
Chemistry
General Medicine
Biodegradation
biology.organism_classification
Pollution
Enzyme assay
Trichloroethylene
Biodegradation, Environmental
Genes, Bacterial
Environmental chemistry
biology.protein
Oxygenases
Water Pollutants, Chemical
Subjects
Details
- ISSN :
- 02697491
- Volume :
- 153
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Environmental pollution (Barking, Essex : 1987)
- Accession number :
- edsair.doi.dedup.....4ea0804451d7775dca1de9a61a76baf6