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Latent 1,3-β-D-glucan acts as an adjuvant for allergen-specific IgE production induced by Japanese cedar pollen exposure
- Source :
- Allergology International, Vol 70, Iss 1, Pp 105-113 (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Background The pollen grains of several plant species contain 1,3-β- D -glucan (BG). BG activates dendritic cells (DCs) and subsequently regulates the innate immune responses. Within Japan, the most common disease associated with type-I hypersensitivity is Japanese cedar pollinosis. However, the role of BG in Japanese cedar pollen (JCP) remains unclear. This study examined the localization and immunological effects of BG in JCP. Methods The localization of BG in JCP grain was determined by immunohistochemical staining using a soluble dectin-1 protein probe and a BG recognition protein (BGRP). The content of BG extracted from JCP was measured by a BGRP-based ELISA-like assay. The cytokine production by bone marrow-derived DCs (BMDCs) obtained from wild-type and BG receptor (dectin-1) knock-out mice was examined in vitro. The mice were intranasally administered JCP grains and the specific serum Ig levels were then quantified. Results BG was detected in the exine and cell wall of the generative cell and tube cell of the JCP grain. Moreover, BG in the exine stimulated production of TNF-α and IL-6 in the BMDCs via a dectin-1-dependent mechanism. Meanwhile, JCP-specific IgE and IgG were detected in the serum of wild-type mice that had been intranasally administered with JCP grains. These mice also exhibited significantly enhanced sneezing behavior. However, dectin-1 knock-out mice exhibited significantly lower JCP-specific IgE and IgG levels compared to wild-type mice. Conclusions Latent BG in JCP can act as an adjuvant to induce JCP-specific antibody production via dectin-1.
- Subjects :
- 0301 basic medicine
lcsh:Immunologic diseases. Allergy
medicine.medical_treatment
Cryptomeria
Cell
Seasonal allergic rhinitis
Immunoglobulin E
Dendritic cells
03 medical and health sciences
Mice
0302 clinical medicine
Adjuvants, Immunologic
C-type lectin
Antibody Specificity
medicine
Immunology and Allergy
Animals
Humans
Receptor
Glucans
Glucan
chemistry.chemical_classification
Innate immune system
biology
Rhinitis, Allergic, Seasonal
General Medicine
Environmental Exposure
Antigens, Plant
Molecular biology
030104 developmental biology
Cytokine
medicine.anatomical_structure
030228 respiratory system
chemistry
Immunoglobulin G
Antibody Formation
biology.protein
Japanese cedar pollen
Pollen
lcsh:RC581-607
Adjuvant
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 13238930
- Volume :
- 70
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Allergology International
- Accession number :
- edsair.doi.dedup.....4e9879a9bd24bc8451ec981a74db4608