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HLA-A, -B, -C, -DRB1, and -DQB1 allele and haplotype frequencies: An analysis of umbilical cord blood units at the Barcelona Cord Blood Bank
- Source :
- HLAREFERENCES. 94(4)
- Publication Year :
- 2019
-
Abstract
- Allele-level HLA compatibility in cord blood transplantation has been associated with better transplant outcomes and is recommended as a selection criterion. It is also a crucial aspect for other therapeutic applications involving cord blood-derived cells. Determination of high-resolution HLA frequencies is an important step towards improving the quality of cord blood banks. We analyzed HLA-A, -B, -C, -DRB1, and -DQB1 allele frequencies in 5458 high-quality cord blood units from the Barcelona Cord Blood Bank and identified 275 class I and 121 class II HLA alleles. A*02:01, B*44:03, C*07:01, DRB1*07:01 and DQB1*03:01 were the most frequent alleles at each locus. We detected 26 novel alleles and were able to determine the presence or absence of some null alleles, including C*04:09N, in a large number of units. We also analyzed maternal HLA typing information for 1877 units to determine real haplotype frequencies and linkage disequilibrium. A*29:02-B*44:03-C*16:01-DRB1*07:01-DQB1*02:02 was the most frequent HLA haplotype and the DRB1-DQB1 gene pair contained the two-locus haplotypes with the strongest linkage disequilibrium values. Four of the 11 unique haplotypes identified in the HLA-homozygous cord blood units were the top-ranking haplotypes identified and were present in 18% of the cohort. This is the first study to report on HLA allele and haplotype frequencies for umbilical cord blood units from the Barcelona Cord Blood Bank and the largest study to date involving two fields of HLA resolution typing of Spanish registry data.
- Subjects :
- Male
Linkage disequilibrium
Genotype
Immunology
Mothers
Human leukocyte antigen
HLA-C Antigens
Biology
Umbilical cord
Polymorphism, Single Nucleotide
Linkage Disequilibrium
HLA-B44 Antigen
Gene Frequency
Pregnancy
HLA-A2 Antigen
Genetics
medicine
Immunology and Allergy
HLA-DQ beta-Chains
Humans
Allele frequency
3' Untranslated Regions
Alleles
HLA-A Antigens
Haplotype
Homozygote
Fetal Blood
Null allele
HLA-A
medicine.anatomical_structure
Haplotypes
HLA-B Antigens
Cord blood
Blood Banks
Female
HLA-DRB1 Chains
Subjects
Details
- ISSN :
- 20592310
- Volume :
- 94
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- HLAREFERENCES
- Accession number :
- edsair.doi.dedup.....4e939f6face1adde1aa3420011696043