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Identification of a Chlamydomonas plastidial 2-lysophosphatidic acid acyltransferase and its use to engineer microalgae with increased oil content
- Source :
- Plant Biotechnology Journal, Plant Biotechnology Journal, 2016, 14 (11), pp.2158-2167. ⟨10.1111/pbi.12572⟩, PLANT BIOTECHNOLOGY JOURNAL(14): 11, Plant Biotechnology Journal, Wiley, 2016, 14 (11), pp.2158-2167. ⟨10.1111/pbi.12572⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- Summary Despite a strong interest in microalgal oil production, our understanding of the biosynthetic pathways that produce algal lipids and the genes involved in the biosynthetic processes remains incomplete. Here, we report that Chlamydomonas reinhardtii Cre09.g398289 encodes a plastid‐targeted 2‐lysophosphatidic acid acyltransferase (CrLPAAT1) that acylates the sn‐2 position of a 2‐lysophosphatidic acid to form phosphatidic acid, the first common precursor of membrane and storage lipids. In vitro enzyme assays showed that CrLPAAT1 prefers 16:0‐CoA to 18:1‐CoA as an acyl donor. Fluorescent protein‐tagged CrLPAAT1 was localized to the plastid membrane in C. reinhardtii cells. Furthermore, expression of CrLPAAT1 in plastids led to a > 20% increase in oil content under nitrogen‐deficient conditions. Taken together, these results demonstrate that CrLPAAT1 is an authentic plastid‐targeted LPAAT in C. reinhardtii, and that it may be used as a molecular tool to genetically increase oil content in microalgae.
- Subjects :
- 0106 biological sciences
0301 basic medicine
lysophosphatidic acid acyltransferase
[SDV]Life Sciences [q-bio]
Chlamydomonas reinhardtii
Plastid membrane
Plant Science
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
triacylglycerols
Plant Oils
Plastids
Plastid
Research Articles
ComputingMilieux_MISCELLANEOUS
biology
microalgae
Chlamydomonas
Phosphatidic acid
biology.organism_classification
Enzyme assay
030104 developmental biology
Biochemistry
chemistry
acyl specificity
Acyltransferases
plastid transformation
oil content
Acyltransferase
biology.protein
Agronomy and Crop Science
010606 plant biology & botany
Biotechnology
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14677644 and 14677652
- Database :
- OpenAIRE
- Journal :
- Plant Biotechnology Journal, Plant Biotechnology Journal, 2016, 14 (11), pp.2158-2167. ⟨10.1111/pbi.12572⟩, PLANT BIOTECHNOLOGY JOURNAL(14): 11, Plant Biotechnology Journal, Wiley, 2016, 14 (11), pp.2158-2167. ⟨10.1111/pbi.12572⟩
- Accession number :
- edsair.doi.dedup.....4e85a24249cff9900cb6ea4a0c396ae5