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Highly Sensitive Bisphenol-A Electrochemical Aptasensor Based on Poly(Pyrrole-Nitrilotriacetic Acid)-Aptamer Film

Authors :
Karine Gorgy
Serge Cosnier
I. Kazane
A. Zazoua
Nicolas Spinelli
Chantal Gondran
Eric Defrancq
Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA)
Département de Chimie Moléculaire (DCM)
Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Département de Chimie Moléculaire - Ingéniérie et Intéractions BioMoléculaires (DCM - I2BM)
Lab LMEPA
Univ Jijel
Source :
Analytical Chemistry, Analytical Chemistry, American Chemical Society, 2016, 88 (14), pp.7268-7273. ⟨10.1021/acs.analchem.6b01574⟩
Publication Year :
2016

Abstract

An electrochemical highly sensitive aptasensor was developed based on electropolymerized poly(pyrrole-nitrilotriacetic) acid film and a new aptamer functionalized by a pentahistidine peptide for the quantification of bisphenol A. A surface coverage of antibisphenol A aptamer of 1.84 × 10(-10) mol cm(-2) was estimated from the electrochemical signal of the [Ru(III)(NH3)6](3+) complex bound by electrostatic interactions onto the aptamer-modified electrode. The binding of bisphenol A onto the polymer film was successfully characterized by electrochemical methods as square wave voltammetry and electrochemical impedance spectroscopy measurements. The designed label-free impedimetric aptasensor displayed a wide linear range from 10(-11) to 10(-6) mol L(-1) with a sensitivity of 372 Ω per unit of log of concentration and an excellent specificity toward interfering agents such as 4,4'-dihydroxybiphenyl and bisphenol P.

Details

ISSN :
15206882 and 00032700
Volume :
88
Issue :
14
Database :
OpenAIRE
Journal :
Analytical chemistry
Accession number :
edsair.doi.dedup.....4e829ca11021917e691dc56e390a1013