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Spin-label saturation-transfer electron spin resonance detection of transient association of rhodopsin in reconstituted membranes
- Source :
- Biochemistry. 21:5978-5983
- Publication Year :
- 1982
- Publisher :
- American Chemical Society (ACS), 1982.
-
Abstract
- Rotational diffusion of rhodopsin in reconstituted membranes of phosphatidylcholines of various alkyl chain lengths has been measured by using saturation-transfer electron spin resonance spectroscopy as a function of temperature and lipid/rhodopsin mole ratio. For dipalmitoyl-phosphatidylcholine, the rotational correlation time is 20 microseconds at physiological concentration, the same as in rod outer segment (ros) membranes. Dilution reduces the time to 10 microseconds, a value that is ascribed to well-dispersed monomeric rhodopsin. Use of phospholipids with longer or shorter chains results in sharply increased rotational correlation times. It is concluded that rhodopsin molecules are transiently associated in both reconstituted and ros membranes and that the nature of the association is determined by lipid type and composition.
- Subjects :
- Rhodopsin
Chemical Phenomena
Rotation
genetic structures
Macromolecular Substances
Membrane Fluidity
Biochemistry
law.invention
Diffusion
Membrane Lipids
Nuclear magnetic resonance
law
Animals
Photoreceptor Cells
Electron paramagnetic resonance
Spin label
Spectroscopy
Alkyl
Rotational correlation time
chemistry.chemical_classification
biology
Cell Membrane
Electron Spin Resonance Spectroscopy
Temperature
Rotational diffusion
Membranes, Artificial
Pulmonary Surfactants
Rod Cell Outer Segment
Chemistry
Membrane
chemistry
Phosphatidylcholines
biology.protein
Biophysics
Cattle
lipids (amino acids, peptides, and proteins)
sense organs
Retinal Pigments
Subjects
Details
- ISSN :
- 15204995 and 00062960
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Biochemistry
- Accession number :
- edsair.doi.dedup.....4e6a6ea4a08509cf4d7d7766a437616d