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Cyclic Peptide-Decorated Self-Assembled Nanohybrids for Selective Recognition and Detection of Multivalent RNAs
- Source :
- Bioconjugate chemistry. 27(3)
- Publication Year :
- 2016
-
Abstract
- Although there has been substantial advancement in the development of nanostructures, the development of self-assembled nanostructures that can selectively recognize multivalent targets has been very difficult. Here we show the proof of concept that topology-controlled peptide nanoassemblies can selectively recognize and detect a multivalent RNA target. We compared the differential behaviors of peptides in a linear or cyclic topology in terms of peptide-gold nanoparticle hybrid nanostructure formation, conformational stabilization, monovalent and multivalent RNA binding in vitro, and multivalent RNA recognition in live cells. When the topology-dependent selectivity amplification of the cyclic peptide hybrids is combined with the noninvasive nature of dark-field microscopy, the cellular localization of the viral Rev response element (RRE) RNA can be monitored in situ. Because intracellular interactions are often mediated by overlapping binding partners with weak affinity, the topology-controlled peptide assemblies can provide a versatile means to convert weak ligands into multivalent ligands with high affinity and selectivity.
- Subjects :
- Pharmacology
chemistry.chemical_classification
010405 organic chemistry
Organic Chemistry
Biomedical Engineering
Pharmaceutical Science
RNA
Nanoparticle
Bioengineering
Peptide
010402 general chemistry
01 natural sciences
Combinatorial chemistry
Peptides, Cyclic
Cyclic peptide
In vitro
0104 chemical sciences
Nanostructures
chemistry
Microscopy, Electron, Transmission
Selectivity
Cellular localization
Intracellular
Biotechnology
Subjects
Details
- ISSN :
- 15204812
- Volume :
- 27
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Bioconjugate chemistry
- Accession number :
- edsair.doi.dedup.....4e27b77d4ba5870814d1eddac1ac2178