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Adsorption equilibrium, kinetics and thermodynamics of α-amylase on poly(DVB-VIM)-Cu(+2) magnetic metal-chelate affinity sorbent
- Source :
- Applied biochemistry and biotechnology. 168(2)
- Publication Year :
- 2011
-
Abstract
- Designing an immobilised metal ion affinity process on large-scale demands that a thorough understanding be developed regarding the adsorption behaviour of proteins on metal-loaded gels and the characteristic adsorption parameters to be evaluated. In view of this requirement, interaction of alpha-amylase as a model protein with newly synthesised magnetic-poly(divinylbenzene-1-vinylimidazole) [m-poly(DVB-VIM)] microbeads (average diameter, 53-212 mu m) was investigated. The m-poly(DVB-VIM) microbeads were prepared by copolymerising of divinylbenzene (DVB) with 1-vinylimidazole (VIM). The m-poly(DVB-VIM) microbeads were characterised by N-2 adsorption/desorption isotherms, electron spin resonance, elemental analysis, scanning electron microscope and swelling studies. Cu2+ ions were chelated on the m-poly(DVB-VIM) beads and used in adsorption of alpha-amylase in a batch system. The maximum alpha-amylase adsorption capacity of the m-poly(DVB-VIM)-Cu2+ beads was determined as 10.84 mg/g at pH 6.0, 25 A degrees C. The adsorption data were analyzed using three isotherm models, which are the Langmuir, Freundlich and Dubinin-Radushkevich isotherm models. The pseudo-first-order, pseudo-second-order, modified Ritchie's-second-order and intraparticle diffusion models were used to test dynamic experimental data. The study of temperature effect was quantified by calculating various thermodynamic parameters such as Gibbs free energy, enthalpy and entropy changes.
- Subjects :
- Langmuir
Biochemistry & molecular biology
Thermodynamic parameter
Entropy
Aspergillus oryzae
Analytical chemistry
Protein adsorption
Applied Microbiology and Biotechnology
Biochemistry
Polymerization
Ima
Diffusion
chemistry.chemical_compound
Electron spin resonance
Desorption
Immobilized Enzymes
Amylases
Storage Stability
Chemical analysis
Metal ions
Dyes
Chelating Agents
Magnetic beads
Biotechnology & applied microbiology
Chelation
Chemistry
Adsorption isotherms
Imidazoles
Temperature
General Medicine
Microspheres
Metals
Magnetic moments
symbols
Magnets
Thermodynamics
Polyvinyls
Beads
Gibbs free energy
Scanning electron microscopy
Divinylbenzene
Biotechnology
Sorbent
Enthalpy
Bioengineering
Article
Styrenes
Adsorption equilibria
Immobilization
Intraparticle diffusion models
symbols.namesake
Adsorption
Metal ion affinities
Freundlich equation
Free energy
Metal chelate
Molecular Biology
Ions
Isotherm
Matrix
Aqueous-solution
Alpha-amylase
Proteins
Water
Adsorption capacities
Amylase
Enzymes, Immobilized
1 vinylimidazole derivative
Kinetics
Zn(II)
Adsorption equilibrium
Physical chemistry
Kinetics and thermodynamics
alpha-Amylases
Removal
Immobilised metal ion affinity
Copper
Model
Subjects
Details
- ISSN :
- 15590291
- Volume :
- 168
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Applied biochemistry and biotechnology
- Accession number :
- edsair.doi.dedup.....4e239d7bf9e2a624058d6e86712ed38b