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A guide toMycobacteriummutagenesis
- Source :
- The FEBS Journal. 286:3757-3774
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- The genus Mycobacterium includes several pathogens that cause severe disease in humans, like Mycobacterium tuberculosis (M. tb), the infectious agent causing tuberculosis. Genetic tools to engineer mycobacterial genomes, in a targeted or random fashion, have provided opportunities to investigate M. tb infection and pathogenesis. Furthermore, they have allowed the identification and validation of potential targets for the diagnosis, prevention, and treatment of tuberculosis. This review describes the various methods that are available for the generation of mutants in Mycobacterium species, focusing specifically on tools for altering slow-growing mycobacteria from the M. tb complex. Among others, it incorporates the recent new molecular biological technologies (e.g. ORBIT) to rapidly and/or genome-wide comprehensively obtain targeted mutants in mycobacteria. As such, this review can be used as a guide to select the appropriate genetic tools to generate mycobacterial mutants of interest, which can be used as tools to aid understanding of M. tb infection or to help developing TB intervention strategies.
- Subjects :
- 0301 basic medicine
Tuberculosis
Mutagenesis (molecular biology technique)
Mycobacterium tuberculosis
Cell Biology
Computational biology
Biology
biology.organism_classification
medicine.disease
Biochemistry
Genome
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Genome editing
Genes, Bacterial
Mutagenesis
030220 oncology & carcinogenesis
medicine
Transposon mutagenesis
Identification (biology)
Molecular Biology
Mycobacterium
Subjects
Details
- ISSN :
- 17424658 and 1742464X
- Volume :
- 286
- Database :
- OpenAIRE
- Journal :
- The FEBS Journal
- Accession number :
- edsair.doi.dedup.....4e1a1ebe461439792bb86091bc6cfe45