Impact of sprouting and cooking on antioxidant compounds and activity in different Italian varieties of Lens culinaris L

Lentils (Lens culinaris L.) are among the most ancient seeds cultivated since prehistory. Several lentil varieties, differing for nutrients and antioxidant compounds, are cultivated in various regions of Italy. Seeds of three varieties of L. culinaris: Onano, Beluga and Villalba, differing for morphology, colour and size, were studied. Antioxidant activity and the content in total polyphenols, flavonoids and flavonols were evaluated in raw and cooked seeds and in cooking water. The same compounds were quantified in sprouts up to 4 days of germination. The highest content of polyphenols was observed in integument of Beluga seeds, flavonoids in integument of Villalba seeds, while flavonols were particularly abundant in cotyledon of Onano seeds. After seed cooking, a decrease in phytochemicals and antioxidant capacity was observed in tissues but they were partially recovered in cooking water. ·