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High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius
- Source :
- Frontiers in Microbiology, Vol 12 (2021)
- Publication Year :
- 2021
- Publisher :
- Frontiers Media S.A., 2021.
-
Abstract
- Significant technical challenges have limited the study of extremophile cell biology. Here we describe a system for imaging samples at 75°C using high numerical aperture, oil-immersion lenses. With this system we observed and quantified the dynamics of cell division in the model thermoacidophilic crenarchaeon Sulfolobus acidocaldarius with unprecedented resolution. In addition, we observed previously undescribed dynamic cell shape changes, cell motility, and cell-cell interactions, shedding significant new light on the high-temperature lifestyle of this organism.
- Subjects :
- Microbiology (medical)
Sulfolobus acidocaldarius
biology
Cell division
Chemistry
Cell
Motility
cytokinesis
microcolony formation
cell motility
biology.organism_classification
Microbiology
live-cell imaging
QR1-502
Sulfolobus
Cell biology
archaeal cell biology
medicine.anatomical_structure
Live cell imaging
medicine
Extremophile
Cytokinesis
Subjects
Details
- Language :
- English
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Microbiology
- Accession number :
- edsair.doi.dedup.....4e01fe38a38a5529e5f04d18ac8fdad5