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A Cathepsin B Inhibitor, E-64, Improves the Preimplantation Development of Bovine Somatic Cell Nuclear Transfer Embryos
- Source :
- The Journal of Reproduction and Development
- Publication Year :
- 2014
- Publisher :
- Japanese Society of Animal Reproduction, 2014.
-
Abstract
- Bovine somatic cell nuclear transfer (SCNT) is an important and powerful tool for basic research and biomedical and agricultural applications, however, the efficiency of SCNT has remained extremely low. In this study, we investigated the effects of cathepsin B inhibitor (E-64) supplementation of culture medium on in vitro development of bovine SCNT embryos. We initially used three concentrations of E-64 (0.1, 0.5, 1.0 μm), among which 0.5 μm resulted in the highest rate of blastocysts production after in vitro fertilization (IVF), and was therefore used for further experiments. Blastocyst development of SCNT embryos in the E-64 treatment group also increased relative to the control. Moreover, the cryosurvival rates of IVF and SCNT blastocysts were increased in E-64 treatment groups when compared with the control. On the other hand, we found that IVF and SCNT blastocysts derived from E-64-treated groups had increased total cell numbers and decreased apoptotic nuclei. Furthermore, assessment of the expression of apoptosis-related genes (Bax and Bcl-xL) in bovine IVF and SCNT blastocysts treated with E-64 by real-time RT-PCR analysis revealed suppressed expression of the pro-apoptotic gene Bax and stimulated expression of the anti-apoptotic gene Bcl-xL. Taken together, these finding indicate that addition of E-64 to embryo culture medium may have important implications for improving developmental competence and preimplantation quality in bovine IVF and SCNT embryos.
- Subjects :
- Nuclear Transfer Techniques
medicine.medical_treatment
Embryonic Development
Apoptosis
Cysteine Proteinase Inhibitors
Biology
Cathepsin B
Embryo Culture Techniques
Andrology
Leucine
medicine
Animals
Embryo Implantation
Blastocyst
reproductive and urinary physiology
In vitro fertilisation
Somatic cell nuclear transfer (SCNT)
Embryo culture
Embryo
Bovine
Molecular biology
In vitro
medicine.anatomical_structure
Cathepsin B inhibitor (E-64)
embryonic structures
Somatic cell nuclear transfer
Original Article
Blastocysts
Cattle
Female
Animal Science and Zoology
Subjects
Details
- ISSN :
- 13484400 and 09168818
- Volume :
- 60
- Database :
- OpenAIRE
- Journal :
- Journal of Reproduction and Development
- Accession number :
- edsair.doi.dedup.....4dcb6880102d3003ab28285772956249