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Role of the sequence of the rne-dependent site in 3′ processing of M1 RNA, the catalytic component ofEscherichia coliRNase P
- Source :
- FEBS Letters. 505:291-295
- Publication Year :
- 2001
- Publisher :
- Wiley, 2001.
-
Abstract
- The 3′ processing of M1 RNA, the catalytic component of Escherichia coli RNase P, occurs by two pathways involving multiple steps. The precursor of M1 RNA has an rne-dependent site downstream of the processing site, whose sequence variation affects the processing efficiency. In this study, we showed that the sequence itself of the rne-dependent site possessed the ability to determine the processing pathways and that it also affected the cleavage specificity with the generation of the processing products at one nucleotide upstream or downstream of the normal cleavage sites.
- Subjects :
- Transcription, Genetic
Rne-dependent site
RNase P
Molecular Sequence Data
Biophysics
M1 RNA
medicine.disease_cause
Biochemistry
Catalysis
Ribonuclease P
Structural Biology
Transcription (biology)
Endoribonucleases
Escherichia coli
Genetics
medicine
RNA, Catalytic
Binding site
Molecular Biology
Messenger RNA
Binding Sites
3′ Processing
Base Sequence
Chemistry
Escherichia coli Proteins
Nucleic acid sequence
RNA
Cell Biology
Blotting, Northern
Molecular biology
RNase MRP
Mutagenesis, Site-Directed
Plasmids
Protein Binding
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 505
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....4dc4dd4afa6bb1f1f64c0f1a970fc888