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EMT Transition Alters Interstitial Fluid Flow–Induced Signaling in ERBB2-Positive Breast Cancer Cells
- Source :
- Molecular Cancer Research. 13:755-764
- Publication Year :
- 2015
- Publisher :
- American Association for Cancer Research (AACR), 2015.
-
Abstract
- A variety of biophysical forces are altered in the tumor microenvironment (TME) and these forces can influence cancer progression. One such force is interstitial fluid flow (IFF)—the movement of fluid through the tissue matrix. IFF was previously shown to induce invasion of cancer cells, but the activated signaling cascades remain poorly understood. Here, it is demonstrated that IFF induces invasion of ERBB2/HER2-expressing breast cancer cells via activation of phosphoinositide-3-kinase (PI3K). In constitutively activate ERBB2-expressing cells that have undergone epithelial-to-mesenchymal transition (EMT), IFF-mediated invasion requires the chemokine receptor CXCR4, a gradient of its ligand CXCL12, and activity of the PI3K catalytic subunits p110α and β. In wild-type ERBB2-expressing cells, IFF-mediated invasion is chemokine receptor–independent and requires only p110α activation. To test whether cells undergoing EMT alter their signaling response to IFF, TGFβ1 was used to induce EMT in wild-type ERBB2-expressing cells, resulting in IFF-induced invasion dependent on CXCR4 and p110β. Implications: This study identifies a novel signaling mechanism for interstitial flow–induced invasion of ERBB2-expressing breast cancer cells, one that depends on EMT and acts through a CXCR4–PI3K pathway. These findings suggest that the response of cancer cells to interstitial flow depends on EMT status and malignancy. Mol Cancer Res; 13(4); 755–64. ©2015 AACR.
- Subjects :
- Receptors, CXCR4
Cancer Research
Epithelial-Mesenchymal Transition
Receptor, ErbB-2
Breast Neoplasms
Biology
CXCR4
Phosphatidylinositol 3-Kinases
Chemokine receptor
Transforming Growth Factor beta
Cell Line, Tumor
Tumor Microenvironment
medicine
Humans
Neoplasm Invasiveness
Epithelial–mesenchymal transition
Molecular Biology
PI3K/AKT/mTOR pathway
Tumor microenvironment
Cancer
Extracellular Fluid
medicine.disease
Oncology
Cancer cell
Cancer research
Female
Signal transduction
Signal Transduction
Subjects
Details
- ISSN :
- 15573125 and 15417786
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Molecular Cancer Research
- Accession number :
- edsair.doi.dedup.....4dbf9434bfe8cdfff85976ea136336c8