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Development of a General-Primer-PCR-Reverse-Line-Blotting System for Detection of Beta and Gamma Cutaneous Human Papillomaviruses
- Source :
- Journal of Clinical Microbiology, 43(11), 5581-5587. American Society for Microbiology, Brink, A A T P, Lloveras, B, Nindl, I, Heideman, D A M, Kramer, D, Pol, R, Fuente, M J, Meijer, C J L M & Snijders, P J F 2005, ' Development of a general-primer-PCR-reverse-line-blotting system for detection of beta and gamma cutaneous human papillomaviruses ', Journal of Clinical Microbiology, vol. 43, no. 11, pp. 5581-5587 . https://doi.org/10.1128/JCM.43.11.5581-5587.2005
- Publication Year :
- 2005
- Publisher :
- American Society for Microbiology, 2005.
-
Abstract
- The beta and gamma genera of papillomaviruses consist of epidermodysplasia verruciformis-related human papillomaviruses (HPVs) and phylogenetically related cutaneous HPVs. Here, we have developed a consensus primer PCR assay and reverse line blot typing system coupled thereto (referred to as beta and gamma cutaneous HPV PCR [BGC-PCR]) for detection and typing of 24 beta and gamma HPVs (HPV types 4, 5, 8, 9, 12, 14, 15, 17, 19, 20, 21, 22, 23, 24, 25, 36, 37, 38, 47, 48, 49, 50, 60, and 65). Because the HPV-specific PCR products are only 72 bp in size, the system is suitable for formalin-fixed, paraffin-embedded specimens and other samples in which the DNA is of suboptimal quality. This system was able to detect and type as little as 100 ag to 1 fg HPV DNA per reaction (depending on the HPV type) in a background of 100 ng human DNA without any cross-reactivity between the tested types. Beta and gamma HPVs were detected in DNA extracted from plucked eyebrow hairs of 31 of 34 renal transplant recipients. In addition, formalin-fixed, paraffin-embedded specimens from nonmelanoma skin tumors of renal transplant recipients ( n = 25) and immunocompetent individuals ( n = 15) scored BGC-PCR positive in 21 and 6 cases, respectively, with HPV type 5 (HPV5) and HPV8 being the predominant types. The data indicate that this method can be a valuable, user-friendly tool for the detection and typing of cutaneous HPV in clinical specimens and may have implications for future monitoring of vaccines or alternative treatment modalities for diseases caused by these cutaneous HPVs.
- Subjects :
- Microbiology (medical)
Biopsy
Molecular Sequence Data
Biology
Polymerase Chain Reaction
Sensitivity and Specificity
law.invention
chemistry.chemical_compound
law
Virology
medicine
Humans
Typing
Papillomaviridae
Beta (finance)
Polymerase chain reaction
DNA Primers
Skin
Base Sequence
medicine.diagnostic_test
virus diseases
biology.organism_classification
Kidney Transplantation
Molecular biology
Blot
chemistry
Warts
Primer (molecular biology)
Immunocompetence
Sequence Alignment
DNA
Hair
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....4da4258a1746e51bccbf9b746bcba9bf