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High-throughput sequencing reveals inbreeding depression in a natural population
- Source :
- Proceedings of the National Academy of Sciences of the U.S.A., 111(10), 3775-3780. National Academy of Sciences, Proceedings of the National Academy of Sciences of the United States of America, 111(10), 3775. National Academy of Sciences
- Publication Year :
- 2014
-
Abstract
- Proxy measures of genome-wide heterozygosity based on approximately 10 microsatellites have been used to uncover heterozygosity fitness correlations (HFCs) for a wealth of important fitness traits in natural populations. However, effect sizes are typically very small and the underlying mechanisms remain contentious, as a handful of markers usually provides little power to detect inbreeding. We therefore used restriction site associated DNA (RAD) sequencing to accurately estimate genome-wide heterozygosity, an approach transferrable to any organism. As a proof of concept, we first RAD sequenced oldfield mice (Peromyscus polionotus) from a known pedigree, finding strong concordance between the inbreeding coefficient and heterozygosity measured at 13,198 single-nucleotide polymorphisms (SNPs). When applied to a natural population of harbor seals (Phoca vitulina), a weak HFC for parasite infection based on 27 microsatellites strengthened considerably with 14,585 SNPs, the deviance explained by heterozygosity increasing almost fivefold to a remarkable 49%. These findings arguably provide the strongest evidence to date of an HFC being due to inbreeding depression in a natural population lacking a pedigree. They also suggest that under some circumstances heterozygosity may explain far more variation in fitness than previously envisaged.
- Subjects :
- 0106 biological sciences
Heterozygote
Population
Restriction Mapping
Genetic Fitness
heterozygosity fitness correlation
Phoca
Biology
RAD sequencing
010603 evolutionary biology
01 natural sciences
Polymorphism, Single Nucleotide
Loss of heterozygosity
03 medical and health sciences
Peromyscus
SDG 3 - Good Health and Well-being
single nucleotide polymorphism
genetic variability
Inbreeding depression
Genetics
Animals
Inbreeding
Genetic variability
Polymorphism
education
030304 developmental biology
0303 health sciences
education.field_of_study
Multidisciplinary
Genetic Variation
High-Throughput Nucleotide Sequencing
Single Nucleotide
Biological Sciences
Genetics, Population
Natural population growth
Evolutionary biology
Microsatellite
Illinois
North Sea
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 111
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....4d9eed02f35845f960ba63e8e35582be