Back to Search
Start Over
Detection of hypermethylated genes as markers for cervical screening in women living with HIV
- Source :
- Kremer, W W, Van Zummeren, M, Novianti, P W, Richter, K L, Verlaat, W, Snijders, P J, Heideman, D A, Steenbergen, R D, Dreyer, G & Meijer, C J 2018, ' Detection of hypermethylated genes as markers for cervical screening in women living with HIV ', Journal of the international aids society, vol. 21, no. 8, pp. e25165 . https://doi.org/10.1002/jia2.25165, Journal of the international aids society, 21(8). International AIDS Society, Journal of the International AIDS Society
- Publication Year :
- 2018
-
Abstract
- INTRODUCTION: To evaluate the performance of hypermethylation analysis of ASCL1, LHX8 and ST6GALNAC5 in physician-taken cervical scrapes for detection of cervical cancer and cervical intraepithelial neoplasia (CIN) grade 3 in women living with HIV (WLHIV) in South Africa.METHODS: Samples from a prospective observational cohort study were used for these analyses. Two cohorts were included: a cohort of WLHIV who were invited for cervical screening (n = 321) and a gynaecologic outpatient cohort of women referred for evaluation of abnormal cytology or biopsy proven cervical cancer (n = 108, 60% HIV seropositive). Cervical scrapes collected from all subjects were analysed for hypermethylation of ASCL1, LHX8 and ST6GALNAC5 by multiplex quantitative methylation specific PCR (qMSP). Histology endpoints were available for all study subjects.RESULTS: Hypermethylation levels of ASCL1, LHX8 and ST6GALNAC5 increased with severity of cervical disease. The performance for detection of CIN3 or worse (CIN3+ ) as assessed by the area under the receiver operating characteristic (ROC) curves (AUC) was good for ASCL1 and LHX8 (AUC 0.79 and 0.81 respectively), and moderate for ST6GALNAC5 (AUC 0.71). At a threshold corresponding to 75% specificity, CIN3+ sensitivity was 72.1% for ASCL1 and 73.8% for LHX8 and all samples from women with cervical cancer scored positive for these two markers.CONCLUSIONS: Hypermethylation analysis of ASCL1 or LHX8 in cervical scrape material of WLHIV detects all cervical carcinomas with an acceptable sensitivity and good specificity for CIN3+ , warranting further exploration of these methylation markers as a stand-alone test for cervical screening in low-resource settings.
- Subjects :
- Adult
0301 basic medicine
medicine.medical_specialty
DNA Methylation Marker Testing
LIM-Homeodomain Proteins
Human immunodeficiency virus (HIV)
Uterine Cervical Neoplasms
HIV Infections
medicine.disease_cause
Cohort Studies
South Africa
03 medical and health sciences
0302 clinical medicine
High‐grade Cervical Intraepithelial Neoplasia
Coenzyme A Ligases
Biomarkers, Tumor
medicine
Humans
Mass Screening
Prospective Studies
Human papillomavirus
Research Articles
Early Detection of Cancer
Cervical screening
Human Immuno‐deficiency Virus
business.industry
European research
Public Health, Environmental and Occupational Health
Human Papillomavirus
DNA, Neoplasm
DNA Methylation
Middle Aged
Uterine Cervical Dysplasia
Human immuno deficiency virus
Sialyltransferases
3. Good health
030104 developmental biology
Infectious Diseases
ROC Curve
030220 oncology & carcinogenesis
Family medicine
High Grade Cervical Intraepithelial Neoplasia
Female
business
Research Article
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 17582652
- Database :
- OpenAIRE
- Journal :
- Kremer, W W, Van Zummeren, M, Novianti, P W, Richter, K L, Verlaat, W, Snijders, P J, Heideman, D A, Steenbergen, R D, Dreyer, G & Meijer, C J 2018, ' Detection of hypermethylated genes as markers for cervical screening in women living with HIV ', Journal of the international aids society, vol. 21, no. 8, pp. e25165 . https://doi.org/10.1002/jia2.25165, Journal of the international aids society, 21(8). International AIDS Society, Journal of the International AIDS Society
- Accession number :
- edsair.doi.dedup.....4d96878faae992988bf4bc2b0ce3ee9e